摘要
目的探讨人胚胎干细胞(hESCs)向胰岛素分泌细胞(IPCs)诱导分化过程中基因表达的差异,并构建miRNA-mRNA调控网络。方法从GEO数据库的GSE42094数据集筛选差异表达基因,对其进行GO功能和KEGG路径显著性分析。选用miRTarBase数据库预测其靶miRNA,应用cytoscape 9.1.0软件构建miRNA-mRNA调控网络并可视化,采用文献数据验证预测的miRNA。结果本研究共筛选得到差异表达的基因188个,分别选取诱导后上调和下调的各前10个进行分析,发现上调基因蛋白互作网络通过VTN与下调基因网络的POU5F1直接作用,将两个网络相关联。miRNA-mRNA调控网络发现miR-335-5p能够调控VTN、NANOG、POU5F1和DNMT3B 4个基因的表达,进一步验证发现miR-335-5p表达量随着诱导分化进程逐渐升高,与NANOG、POU5F1和DNMT3B表达量逐渐降低形成对照,提示二者之间存在靶向关系且为负性调控。结论miRNA-mRNA调控网络显示miR-335-5p靶向多个基因参与hESCs向IPCs诱导分化过程。
Objective To investigate the difference of gene expression during the differentiation of human embryonic stem cells(hESCs)into insulin producing cells(IPCs),and to construct a miRNA-mRNA regulatory network.Methods Differentially expressed genes were screened from the GSE42094 dataset of the GEO database,and GO function and KEGG pathway significance analysis were performed.The miRTarBase database was used to predict its target miRNA,and the miRNA-mRNA regulatory network was constructed and visualized by cytoscape 9.1.0 software.The predicted miRNA was verified by literature data.Results In this study,188 differentially expressed genes were screened,and the top 10 up-regulated and down-regulated genes after induction were selected for analysis.It was found that the up-regulated gene protein interaction network directly interacted with the POU5F1 of the down-regulated gene network through VTN,and the two networks were associated.The miRNA-mRNA regulatory network found that miR-335-5p could regulate the expression of VTN,NANOG,POU5F1 and DNMT3B.Further verification found that the expression of miR-335-5p gradually increased with the induction of differentiation,and the expression of NANOG,POU5F1 and DNMT3B gradually decreased to form a control,suggesting that there was a targeted relationship between the two and negative regulation.Conclusion The miRNA-mRNA regulatory network shows that miR-335-5p targets multiple genes to participate in the differentiation of hESCs into IPCs.
作者
王涛
侯润博
潘鑫
WANG Tao;HOU Run-bo;PAN Xin(Department of Medicine of Medical Treatment College,Jinzhou Medical University,Jinzhou 121013,Liaoning,China;Medical Radiologic Technology of Medical Treatment College,Jinzhou Medical University,Jinzhou 121013,Liaoning,China)
出处
《医学信息》
2023年第6期8-13,共6页
Journal of Medical Information
基金
辽宁省教育厅科学研究经费面上项目(编号:LJKZ1374)
辽宁省大学生创新训练项目(编号:S202113213014)。
