摘要
【目的】分析确定黄柏、石菖蒲合剂抗炎作用的药效物质基础,并确定可能的作用靶点。【方法】通过小鼠耳廓肿胀试验检测黄柏、石菖蒲合剂的抗炎作用,通过网络药理学方法分析其与炎症相关蛋白的互作网络图,采用Cytoscape 3.6.1软件绘制药物-活性成分-疾病-共同靶点网络图,并进行GO功能和KEGG通路富集分析,应用AutoDock软件将主要活性成分与核心靶点进行分子对接。【结果】黄柏、石菖蒲合剂对二甲苯所致小鼠耳廓肿胀的抑制率为56.96%,与模型组差异显著(P<0.05)。通过网络药理学分析共获得30个黄柏、石菖蒲合剂性化合物,包括槲皮素、小檗碱、山柰酚等;获得25个黄柏、石菖蒲合剂与炎症交集靶点,包括白细胞介素-6(IL6)、IL1β、丝氨酸/苏氨酸蛋白激酶(AKT1)等;GO功能富集结果显示,生物过程、细胞组分和分子功能分别获得334、13和27个条目,其中生物过程涉及正调控一氧化氮生物合成过程、正调控凋亡过程、正调控平滑肌细胞增殖、炎症反应等;细胞组分涉及细胞外间隙、胞外区、分泌颗粒等;分子功能涉及细胞因子活性、酶结合、RNA聚合酶Ⅱ转录因子活性、配体激活序列特异性DNA结合等方面。KEGG结果分析得到82条通路,发现黄柏、石菖蒲合剂抗炎与TNF信号通路、IL17信号通路、脂质和动脉粥样硬化等可能有一定关系。【结论】黄柏、石菖蒲合剂具有较好的抗炎效果,其抗炎机制可能涉及多种活性成分对不同信号通路基因和蛋白表达的调节,说明黄柏、石菖蒲合剂是通过多成分、多通路、多靶点发挥抗炎作用。
【Objective】This study was aimed to analyze and determine the pharmacodynamic material basis of anti-inflammatory effect of Phellodendron amurense and Acorus calamus mixture,and determine the possible target.【Method】The anti-inflammatory effect of Phellodendron amurense and Acorus calamus mixture was tested by auricular swelling test in mice,and then the interaction(PPI)network diagram with inflammation-related proteins was analyzed by network pharmacology.Cytoscape 3.7.2 software was used to draw the network diagram of drug-active component-disease-common target,and GO function and KEGG pathway were enriched and analyzed.Autodock software was used to connect the main active ingredients with the core target.【Result】The inhibition rate of auricular swelling induced by xylene in mice by Phellodendron amurense and Acorus calamus mixture was 56.96%,which was significantly different from the model group(P<0.05).Through network pharmacology analysis,a total of 30 active compounds of Phellodendron amurense and Acorus calamus mixture such as quercetin,berberine,and kaempferol were obtained,and 25 intersection targets of Phellodendron amurense and Acorus calamus mixture and inflammation were interleukin-6(IL6),IL1β,and serine/threonine protein kinase(AKT1).GO enrichment analysis results showed that a total of 334 biological processes,13 cellular components,and 27 molecular functions were obtained.Biological processes involved positive regulation of nitric oxide biosynthesis process,positive regulation of apoptotic process,positive regulation of smooth muscle cell proliferation,and inflammatory response.Cellular components involved the extracellular space,extracellular region,and secretory granules.Molecular functions involved cytokine activity,enzyme binding,RNA polymeraseⅡtranscription factor activity,and ligand-activated sequence-specific DNA binding.KEGG results analysis yielded 82 pathway,and it was found that there might be some relationship between anti-inflammatory effects of Phellodendron amurense and Acorus calamus mixture and TNF signaling pathway,IL17 signaling pathway,lipids and atherosclerosis.【Conclusion】Phellodendron amurense and Acorus calamus mixture had a good anti-inflammatory effect,and its anti-inflammatory mechanism might involve the regulation of gene and protein expression of different signaling pathways by multiple active components,indicating that Phellodendron amurense and Acorus calamus mixture exerted anti-inflammatory effects through multiple components,multiple pathways and multiple targets.
作者
梁天
王明迪
孙鹏
朱爱臣
赵千惠
霍书英
LIANG Tian;WANG Mingdi;SUN Peng;ZHU Aichen;ZHAO Qianhui;HUO Shuying(College of Veterinary Medicine,Hebei Agricultural University,Baoding 071001,China)
出处
《中国畜牧兽医》
CAS
CSCD
北大核心
2023年第3期1195-1206,共12页
China Animal Husbandry & Veterinary Medicine
基金
河北农业大学科研发展基金(JY2018005)。
关键词
炎症
黄柏
石菖蒲
合剂
网络药理学
信号通路
inflammation
Phellodendron amurense
Acorus calamus
mixture
network pharmacology
signaling pathway
