摘要
基于气相色谱法和超高效液相色谱-四极杆-飞行时间串联质谱法探究饲喂裂殖藻粉(Schizochytrium spp.)对山羊乳脂肪酸组成及全脂质组成的影响,并与市售纯牛乳和二十二碳六烯酸(docosahexaenoic acid,DHA)牛乳进行差异性分析。结果表明,山羊乳脂肪含量显著高于牛乳,肉豆蔻酸、棕榈酸和油酸是乳中主要的脂肪酸组成。饲喂裂殖藻粉能显著增加山羊乳中DHA含量。甘油三酯是2种乳中主要的脂质成分,山羊乳中磷脂含量显著高于牛乳。DHA在乳品脂质中主要以甘油酯型DHA形式存在,山羊乳较牛乳含更丰富的磷脂型DHA。裂殖藻粉添加量50 g/d为山羊乳中DHA富集的最佳添加量。通过在日粮中添加DHA原料,能够进一步提高山羊乳的营养价值,解决目前膳食DHA来源单一的问题。本研究为婴幼儿配方奶粉及富DHA功能性乳脂产品的开发和营养调控提供有力的理论依据和参考,并为乳制品类型的鉴定提供方法与数据基础。
In this study,gas chromatography(GC)and ultra-high performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS)were used to explore the effects of feeding Schizochytrium spp.on the fatty acid and lipid composition of goat milk,and the differences in fatty acid and lipid composition between goat milk and commercially available pure bovine milk and docosahexaenoic acid(DHA)-enriched bovine milk were analyzed.The results showed that the lipid content was significantly higher in goat milk than in bovine milk(P<0.05),the major fatty acids in both goat and bovine milk were myristic acid,palmitic acid and oleic acid.The content of DHA in goat milk was significantly increased by feeding Schizochytrium spp..Triglycerides were the major lipid components in goat and bovine milk,and the phospholipid content in goat milk was significantly higher than that in bovine milk.DHA was mainly present in an glyceride esterified form in dairy milk lipids,and goat milk contained more phospholipid(PL)-bound DHA than bovine milk.Feeding Schizochytrium spp.at 50 g/day provided the highest DHA enrichment in goat milk.In a nutshell,this study demonstrated that dietary DHA supplementation can improve the nutritional value of goat milk,thereby solving the problem of the single source of dietary DHA.
作者
冉世前
朱云芬
王杰
李栋
陈潇飞
吕昕
向极钎
陈洪
魏芳
RAN Shiqian;ZHU Yunfen;WANG Jie;LI Dong;CHEN Xiaofei;LÜ Xin;XIANG Jiqian;CHEN Hong;WEI Fang(Oil Crops and Lipids Process Technology National&Local Joint Engineering Laboratory,Key Laboratory of Oilseeds Processing,Ministry of Agriculture and Rural Affairs,Hubei Key Laboratory of Lipid Chemistry and Nutrition,Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,China;Enshi Autonomous Prefecture Academy of Agricultural Sciences,Hubei Selenium Industrial Technology Research Institute,Enshi 445002,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2023年第6期227-234,共8页
Food Science
基金
国家自然科学基金青年科学基金项目(3200161849)
武汉市科技计划项目(2019020701011468)
中国农业科学院创新工程项目(CAAS-ASTIP-2013-OCRI)
油料脂质化学与营养湖北省重点实验室开放基金资助项目(202106)。
