摘要
改良的多平台水环境法(modified multi-platform method,MMPM)由单平台及多平台水环境法发展而来,目前多用于制备睡眠剥夺(sleep deprivation,SD)动物模型。与单平台和早期的多平台水环境相比,MMPM可扩大动物的活动范围,减少群体分离和社会孤立带来的应激,具有易行性和适用性高及造模死亡率低的优点,是国内外较常用的一种SD造模方法。不过,影响SD模型制备的实验因素较多,MMPM在不同研究中的造模效果参差不齐。为提高模型的稳定性和实验的可重复性,本文梳理了MMPM在SD动物模型中的具体应用和评价方法,以供后期相关研究参考。
The modified multi⁃platform method(MMPM)is developed from single⁃platform and multi⁃platform method and is mostly used to prepare sleep deprivation(SD)animal models.Compared with single⁃platform and early multi⁃platform method,MMPM expands the range of activities that animals partake in and decreases the stress caused by group separation and social isolation.Thus,MMPM can increase the social stability of SD animals.The advantages of MMPM,high simplicity and applicability and low mortality,contribute to the popularity in preparing the animal model of SD in China and other countries.However,many experimental factors affect the modeling effects of MMPM,resulting in uneven modeling effects in different SD studies.In this article,to improve the stability and reproductivity of the experimental SD and provide a reference for further,the specific application and evaluation method of MMPM in SD animal models are reviewed.
作者
刁华琼
张婧
王敏
陈雨菲
朱庆生
李小黎
DIAO Huaqiong;ZHANG Jing;WANG Min;CHEN Yufei;ZHU Qingsheng;LI Xiaoli(Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China;Third Hospital of Beijing Chaoyang District,Beijing 100121)
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2023年第1期120-128,共9页
Acta Laboratorium Animalis Scientia Sinica
基金
北京市朝阳区科技计划项目(CYSF2205)。
关键词
改良的多平台水环境法
睡眠剥夺
动物模型
综述
modified multi⁃platform method,MMPM
sleep deprivation
animal models
review
