摘要
优良菌与土著菌的相互作用关系是影响生物强化降解石油烃效果的关键因素。前期研究发现,嗜油菌NY3生物膜对2 g/L含油废水中石油烃的降解率可保持在83%~95%,处理阶段与恢复阶段生物膜上NY3菌及FF菌的生物量存在此消彼长的变化趋势,本文旨在探明该变化趋势的产生机理。研究结果表明:NY3菌对长链烷烃(C30~C34)的降解率高于FF菌,FF菌对中长链烷烃(C13~C29)、蒽、菲的降解率高于NY3菌;NY3菌在中性、弱碱性和低盐度环境下对石油烃的降解率高于酸性和高盐度环境,FF菌在中性、弱酸性、高盐度环境下对石油烃的降解率高于碱性和低盐度环境;NY3菌分泌的鼠李糖脂和绿脓菌素可促进FF菌的生长,但FF菌分泌的海藻糖脂可抑制NY3菌的生长。据此可知,NY3菌与FF菌能协同降解石油烃,但因胞外分泌物对NY3菌和FF菌的作用不同,致使NY3菌和FF菌的生物量出现此消彼长的变化趋势。
Interactions between high-efficient bacteria and indigenous bacteria is a key factor of bioaugmentation.Treatment of oil-containing wastewater by immobilized oleophilic strain NY3 can degrade 83%~95%petroleum hydrocarbons.In the treatment process,biomass of strain NY3 decreased while that of indigenous strain FF increased;in the subsequent recovery process,biomass of strain NY3 increased while that of indigenous strain FF decreased.The aim of this work is to illustrate the variation mechanism.The experiment results suggest that degradation efficiency of long-chain alkane C30〜C34 by strain NY3 is higher than that of strain FF,while degradation efficiency of medium-long chain alkane C13~C29,anthracene and phenanthrene by strain FF is higher than that of strain NY3;strain NY3 grows better in neutral and weakly alkaline environment,and strain FF grows better in neutral and acidic environment;the resistance to salinity of strain FF is higher than that of strain NY3;the extracellular secretions including rhamnolipids and pyocyanin produced by strain NY3 can increase the growth of strain FF,while extracellular trehalose lipids secreted by stain FF can decrease the growth of strain NY3.The results indicate that strain NY3 and strain FF synergisticly degrade oil contaminants by its complementarity from the aspect of the metabolism of petroleum hydrocarbons and degradation environment.Extracellular secretions results in the biomass of the two strains waxes and wanes.In conclusion,we revealed the mechanism of the dominant strain changes during treatment of oil-containing wastewater by immobilized strain NY3 in open system,which may supply a theoretical direction for bioaugmentation o£oil polluted site using strain NY3.
作者
陈婧
聂红云
聂麦茜
王磊
陈丽娇
严寒
林莹莹
CHEN Jing;NIE Hongyun;NIE Maiqian;WANG Lei;CHEN Lijiao;YAN Han;LIN Yingying(School of EnvironmentaI and Municipal Engineering,Xiyan University of Architecture and Technology,Xi'an 710055,China;Key Laboratory of Membrane Separation of Shaanxi Province,Xi'an 710055,China)
出处
《安全与环境工程》
CAS
CSCD
北大核心
2023年第2期173-179,共7页
Safety and Environmental Engineering
基金
陕西省科技计划重点创新产业链项目(2019ZDLSF05-04)
中国博士后科学基金项目(2018M633479)
陕西省科技厅自然科学基础研究计划项目(2019JQ-759)。
关键词
嗜油菌NY3
土著菌FF
石油烃降解
生物强化
协同机理
oleophilic strain NY3
indigenous strain FF
degradation o£petroleum hydrocarbon
bioaugmentation
synergistic mechanism
