摘要
本研究介绍了7例难度较大水生动物采用活体短期注射法快速制备染色体的技术。以7例难度较大水生动物为材料,采用活体注射植物血球凝集素(PHA)和秋水仙素,空气干燥法制备染色体。结果表明:不同类的水生动物,注射部位、注射浓度、间隔时间不同,获得较多分裂相的组织(部位)不同。本研究列出适合7类水生动物体外染色体快速制备方法。该方法具有组织特异性,不需无菌操作,设备简单、操作简便;快速(2~4 h);易于掌握;稳定性好、重复性高;效果良好,可供水生动物染色体快速制备参考。制备的染色体具备进行核型分析、染色体显带处理、性别鉴定及荧光原位杂交(FISH)分析实验分析等优点。
This paper introduced 7 cases of aquatic animals with relatively high difficulty using short-term injection method for chromosome rapid preparation.Methods:7 cases of aquatic animals with great difficulty were used as materials to prepare chromosomes by in vivo injection of PHA and colchicine and air drying.The results showed that the optimal injection sites were different for different aquatic animals with different the injection sites,concentrations and the interval time.This paper listed the best conditions suitable for each aquatic animal,and established a rapid method for the preparation of aquatic animals'chromosomes in vitro.Conclusion:This method has some advantages,such as tissue-specificity,no need for aseptic operation,simple equipment,simple operation,rapid(2-4h),easy to manage,good stability,repeatability and good effect,which can be used for the rapid preparation of all aquatic animal chromosomes.The prepared chromosomes can be used for karyotype analysis,chromosome banding treatment,sex identification and FISH analysis.
作者
张林
何力
张浪
周剑光
甘金华
ZHANG Lin;HE Li;ZHANG Lang;ZHOU Jianguang;GAN Jinhua(Freshwater Fish Germplasm Surveillance and Testing Center,Ministry of Agricul ture,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Wuhan 430223,China)
出处
《中国渔业质量与标准》
2023年第1期19-25,共7页
Chinese Fishery Quality and Standards
基金
中央级公益性科研院所基本科研业务费专项(YFI202202)
中国水产科学研究院基本科研业务费专项(2018JBF13)
中国水产科学研究院基本科研业务费专项(2020TD74)。
