摘要
目的结合体内外实验研究壮药痛风立安胶囊对痛风性关节炎的抗炎作用及机制。方法将60只大鼠随机分为正常组、模型组、阳性对照组(27 mg/kg别嘌醇+0.27 mg/kg秋水仙碱)和痛风立安胶囊低、中、高剂量组(2.2、4.5、9.0 g/kg),每组10只。除正常组外,其余各组大鼠均诱导痛风性关节炎模型。各给药组大鼠灌胃相应药物,正常组和模型组大鼠灌胃等体积纯水,连续14 d。检测大鼠踝关节肿胀率、血清中白细胞介素1β(IL-1β)水平、滑膜组织中核因子κB(NF-κB)蛋白表达水平,观察大鼠踝关节滑膜组织病理形态学变化。另以脂多糖刺激RAW264.7细胞建立炎症模型,以痛风立安胶囊(62.5、125、250μg/mL)干预后,检测细胞中一氧化氮(NO)、活性氧(ROS)、IL-1β水平,NF-κB蛋白表达水平及其在细胞内的定位。结果体内实验结果显示,与正常组比较,模型组大鼠踝关节肿胀率、血清中IL-1β水平、滑膜组织中NF-κB蛋白表达水平均显著升高(P<0.05),表现出滑膜增生、水肿、血管充血、毛细血管增生、炎症细胞增多等病理改变;与模型组比较,痛风立安胶囊高剂量组大鼠上述指标水平均显著降低(P<0.05),中、低剂量组大鼠上述大部分指标水平均显著降低(P<0.05),且大鼠踝关节滑膜增生改善、炎症细胞浸润减少。体外实验结果显示,痛风立安胶囊可显著降低细胞中NO、ROS、IL-1β水平和NF-κB蛋白表达水平(P<0.01),并抑制NF-κB入核。结论痛风立安胶囊对痛风性关节炎具有较好的抗炎作用,其作用机制可能与抑制NF-κB信号通路活性有关。
OBJECTIVE To investigate the anti-inflammatory effects and mechanism of Zhuang medicine Tongfeng li’an capsules on gouty arthritis in combination with in vivo and in vitro experiments.METHODS Sixty rats were randomly divided into normal group,model group,positive control group(27 mg/kg allopurinol+0.27 mg/kg colchicine),Tongfeng li’an capsules lowdose,medium-dose and high-dose groups(2.2,4.5,9.0 g/kg),with 10 rats in each group.Except for normal group,gouty arthritis model of rats was induced in other groups.Rats in each administration group were given corresponding drugs intragastrically,and rats in the normal group and model group were given equal volume of water intragastrically for 14 consecutive days.The degree of ankle joint swelling,serum level of interleukin-1β(IL-1β)and protein expressions of nuclear factor kappa-B(NF-κB)in synovial tissue were detected,and the histopathological changes of synovium tissue in the ankle joint of rats were observed.The inflammation model was established by stimulating RAW264.7 cells with lipopolysaccharide.After Tongfeng li’an capsules(62.5,125,250μg/mL)were given,the levels of nitric oxide(NO),reactive oxygen species(ROS)and IL-1βin the cells and protein expression of NF-κB were detected,and NF-κB localization in the cells was also determined.RESULTS Results of in vivo experiment showed that compared with normal group,the swelling degree of the ankle joint,serum IL-1βlevel and protein expression of NF-κB in synovium tissue were all increased significantly in model group(P<0.05);pathological changes such as synovial hyperplasia,edema,vascular congestion,capillary hyperplasia,and increased inflammatory cells were observed.Compared with model group,the levels of above indexes were all decreased significantly in Tongfeng li’an capsules high-dose group(P<0.05),and most of the above indexes were significantly reduced in Tongfeng li’an capsules medium-dose and low-dose groups(P<0.05);synovial hyperplasia of the ankle joint improved,and the infiltration of inflammatory cells decreased.Results of in vitro experiment showed that Tongfeng li’an capsule could significantly reduce the levels of NO,ROS and IL-1βand protein expression of NF-κB(P<0.01),and inhibit NF-κB nucleation.CONCLUSIONS Tongfeng li’an capsules have good anti-inflammatory effect on gouty arthritis,and its mechanism may be related to the inhibition of NF-κB signaling pathway activity.
作者
赵湘培
杜娜娜
李凤珍
舒建龙
龙朝阳
邹晓素
孙宗喜
ZHAO Xiangpei;DU Nana;LI Fengzhen;SHU Jianlong;LONG Chaoyang;ZOU Xiaosu;SUN Zongxi(Laboratory of Zhuang and Yao Medical Research,Guangxi International Zhuang Medicine Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 530201,China)
出处
《中国药房》
CAS
北大核心
2023年第7期814-818,共5页
China Pharmacy
基金
国家中医药管理局“十二五”重点学科民族药学(壮药学)建设项目
广西中医药大学“高层次人才培育创新团队”项目(No.2022A008)
广西中医药大学博士科研启动基金项目(No.2019BS044)。
关键词
壮药
痛风立安胶囊
痛风性关节炎
抗炎作用
NF-ΚB信号通路
Zhuang medicine
Tongfeng li’an capsule
gouty arthritis
anti-inflammatory effect
NF-κB signaling pathway
