摘要
基于氨氮与纳氏试剂进行显色反应的原理,建立反应体系为1 mL的小体系淡水中氨氮的测定方法。新方法的显色剂用量为20μL、显色时间为10~30 min、盐度<0.5%、pH值为3~11,纳氏试剂以6000 r/min、5 min进行离心处理,采用酶标仪96孔板在420 nm波长下测定显色反应溶液的吸光度。将该方法与《水质氨氮的测定纳氏试剂分光光度法》(HJ 535—2009)(国标法)测定氨氮的吸光度进行正交验证,结果表明2种方法具有良好的拟合度。新方法的检测范围从国标法的0~2.0 mg/L提升到0~4.8 mg/L。方法测定淡水中氨氮的质量浓度具有简便、连续、快速、高批量的优点,适用于实地、实时地测定淡水中的氨氮。
Based on the principle of color reaction between ammonia nitrogen and Nessler s reagent,a method for the determination of ammonia nitrogen in fresh water was established with a reaction system of 1 mL.The dosage of developer was 20μL,the color development time was 10~30 min,salinity was less than 0.5%,pH value was 3~11,and Nessler s reagent was centrifuged at 6000 r/min for 5 min in this method.The absorbance of the color reaction solution was measured at 420 nm by using the 96-well plate of the enzyme marker.The orthogonal validation was carried out between the absorbance of the new method and the absorbance of ammonia nitrogen determined in Water Quality-Determination of Ammonia Nitrogen-Nessler s Reagent Spectrophotometry(HJ 535—2009)(national standard method).The result indicated that the new method showed a good fit with the national standard method.Moreover,the detection range was i ncreased from 0~2.0 mg/L in the national standard method to 0~4.8 mg/L in the new method.The new method for the determination of ammonia nitrogen in fresh water showed the advantages of simplicity,continuity,rapidity and high volume,which was suitable for the field and real-time determination of ammonia nitrogen in fresh water.
作者
卢勇杰
孙巍
李嘉敬
陈心如
邝春议
朱颖
夏春雨
LU Yong-jie;SUN Wei;LI Jia-jing;CHEN Xin-ru;KUANG Chun-yi;ZHU Ying;XIA Chun-yu(School of Biotechnology and Food Engineering,Guangdong University of Petrochemical Technology,Maoming,Guangdong 525000,China)
出处
《环境监控与预警》
2023年第2期56-61,共6页
Environmental Monitoring and Forewarning
基金
国家自然科学青年基金项目(42006137)
广东省基础与应用基础研究基金项目(2022A1515010539)
广东石油化工学院科研基金人才引进项目(2019rc108)
高位池海水养虾尾水无害化处理示范项目
广东省重点领域研发计划项目(2018B020205002)
广东石油化工学院2021年度教育教学改革研究项目(2021JY29)
大学生创新训练项目(73321101,73322049,73322094)。
关键词
淡水
氨氮
小体系
纳氏试剂分光光度法
酶标仪
Fresh water
Ammonia nitrogen determination
Small system
Nessler reagent spectrophotometry
Enzyme-labeling instrument
