摘要
目的:研究加味苓桂术甘汤联合热量限制对apoE^(-/-)小鼠动脉粥样硬化的影响。方法:C57BL/6小鼠为正常对照组,普通饲料喂养,apoE^(-/-)小鼠随机分成模型组、加味苓桂术甘汤组、单纯限食组、中药限食组,其中模型组与加味苓桂术甘汤组以高脂饲料喂养,单纯限食组、中药限食组每日按模型组50%热量投饲高脂饲料,中药限食组和加味苓桂术甘汤组给予加味苓桂术甘汤液2 mL灌胃,其余各组予以等量生理盐水灌胃。12周后,采血及留取胸主动脉,分别采用酶联免疫吸附试验(ELISA)检测血脂、炎症介质水平,苏木精-伊红(HE)染色观察管腔内粥样斑块,蛋白质印迹法(Western Boltting)检测血脂、炎症介质及主动脉腺苷一磷酸活化蛋白激酶、磷酸化腺苷一磷酸活化蛋白激酶、过氧化物酶体增殖物激活受体α、核因子κB、p-核因子κB蛋白表达。结果:与模型组比较,加味苓桂术甘汤组、单纯限食组与中药限食组动脉粥样硬化病变减轻,斑块面积与管腔面积比值明显降低(P<0.05或P<0.01),血清总胆固醇、三酰甘油、低密度脂蛋白胆固醇、肿瘤坏死因子-α、白细胞介素-6及白细胞介素-1β浓度均显著低于模型组(P<0.05或P<0.01),而中药限食组动脉粥样硬化病变最轻,斑块面积与管腔面积比值最低。蛋白质免疫印迹法显示中药限食组磷酸化腺苷一磷酸活化蛋白激酶、过氧化物酶体增殖物激活受体α蛋白表达显著升高(P<0.01),而p-核因子κB蛋白表达显著下降(P<0.01)。结论:加味苓桂术甘汤联合热量限制具有抗动脉粥样硬化作用,其机制可能是通过降低血脂、减轻炎症反应,激活腺苷一磷酸活化蛋白激酶/过氧化物酶体增殖物激活受体α信号通路,从而抑制核因子κB活性,有效发挥抗动脉粥样硬化作用。
Objective:To explore the effects of Modified Ling-Gui-Zhu-Gan Decoction combined with calorie restriction on atherosclerosis of apoE^(-/-)mice.Methods:C57BL/6 mice fed with general fodder were selected as the normal control group(Group A);apoE^(-/-)mice fed with high-fat diet were randomly divided into the model group(Group B),Modified Ling-Gui-Zhu-Gan Decoction(LGZG)group(Group C),calorie restriction group(Group D)and Modified Ling-Gui-Zhu-Gan Decoction combined with calorie restriction group(Group E).Of these groups,Groups B and C were fed with high-fat diet,Groups D and E were fed with 50%calorie restricted high-fat diet as compared with mice in Group B,Groups C and E were administrated with 2 mL LGZG decoction by gavage,and other groups were given the same amount of normal saline by gavage.After 12 weeks,blood was collected and the thoracic aorta was retained,plasma lipid and inflammatory cytokine levels were detected by enzyme linked immunosorbent assay(ELISA),atherosclerotic plaque in the lumen was observed by hematoxylin-eosin(HE)staining,and AMPK,p-AMPK,PPARα,NF-κB and p-NF-κB protein expressions in the plasma lipid,inflammatory cytokine and aorta were measured by Western Blotting.Results:Compared with Group B,Groups C,D and E demonstrated more alleviative atherosclerotic lesions with obvious decrease of the ratio of plaque area to luminal area(P<0.05 or P<0.01);serum TC,TG,LDL-c,TNF-α,IL-6 and IL-1βconcentrations were significantly lower than those in Group B(P<0.05 or P<0.01);minimal atherosclerotic lesions were observed with the minimal ratio of plaque area to luminal area in Group E The results of Western Blot showed that the protein levels of p-AMPK and PPARαin Group E significantly increased(P<0.01),while those of p-NF-κB was significantly reduced(P<0.01).Conclusion:LGZG Decoction combined with calorie restriction has anti-atherosclerotic effects,whose mechanism may be that the AMPK/PPARαsignaling pathway is activated by reducing the plasma lipid and inflammatory reaction,thereby inhibiting the activity of NF-κB and effectively playing the anti-atherosclerotic effects.
作者
张俊杰
李志超
彭国顺
赵晓东
林红军
ZHANG Junjie;LI Zhichao;PENG Guoshun;ZHAO Xiaodong;LIN Hongjun(Zhuhai Hospital of Integrated Traditional Chinese&Western Medicine,Zhuhai 519000,China;Zhongshan Hospital of Traditional Chinese Medicine,Zhongshan 528400,China)
出处
《世界中医药》
CAS
2023年第3期314-319,共6页
World Chinese Medicine
基金
广东省中医药局科研项目(20201323)。
