摘要
旨在研究低氧环境下不同浓度菊苣酸(chicoric acid,CA)对SD大鼠心肌组织低氧适应性的影响。选取60只6~8周龄、体质量为(192.00±7.35)g的健康雄性SD大鼠(P<0.05),随机分为对照组、不同浓度CA组(10,20,40 mg/kg),每组15只,连续灌胃49 d。灌胃50 d时采集SD大鼠心脏,并测定各组大鼠心肌组织活性氧(ROS)含量,抗氧化指标超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化物酶(CAT)活性,线粒体功能相关指标线粒体呼吸链复合体Ⅰ、Ⅲ活性,能量代谢相关指标Na^(+)-K^(+)-ATP酶、Ca^(2+)-ATP酶活性及低氧适应基因缺氧诱导因子1α(HIF-1α)、促红细胞生成素(EPO)、血管内皮生长因子(VEGF)的mRNA及蛋白表达情况。结果显示:与对照组相比,不同浓度CA组显著降低ROS活性(P<0.05);显著降低MDA含量(P<0.05),显著提高SOD、GSH-Px、CAT活性(P<0.05);显著提高线粒体呼吸链复合体Ⅰ、Ⅲ活性(P<0.05);显著提高Na^(+)-K^(+)-ATP酶、Ca^(2+)-ATP酶活性(P<0.05);显著提高HIF-1α、EPO、VEGF的mRNA及蛋白表达(P<0.05)。各试验组间结果显示,40 mg/kg组显著降低ROS活性(P<0.05),显著降低MDA含量(P<0.05),显著提高SOD活性(P<0.05),极显著提高GSH-Px、CAT活性(P<0.01);20,40 mg/kg组极显著提高呼吸链复合体Ⅲ活性(P<0.01);40 mg/kg组极显著提高Na^(+)K^(+)-ATP酶活性(P<0.01),显著提高Ca^(2+)-ATP酶活性(P<0.05);40 mg/kg组极显著促进HIF-1α、EPO的mRNA表达(P<0.01),显著促进HIF-1α、EPO的蛋白表达(P<0.05)。结果表明,CA可通过抑制SD大鼠心肌组织ROS活性,提高其抗氧化能力、线粒体功能、能量代谢水平及低氧适应能力,增强SD大鼠心肌组织低氧适应性,且以40 mg/kg组作用最佳。
This experiment was conducted to investigate the effect of different concentrations of cichoric acid(CA)on hypoxic adaptability of myocardial tissue in SD rats under hypoxic environment.Sixty healthy male SD rats aged 6 to 8 weeks(192.00±7.35)g were selected,SD rats were randomly divided into the control group,different concentrations of CA groups including 10 mg/kg group,20 mg/kg group,40 mg/kg group,15 mg/kg group,continuously feeding for 49 d.Feeding of the 50th day,the hearts of SD rats were collected,the content of reactive oxygen species(ROS),antioxidant indexes superoxide dismutase(SOD),malondialdehyde(MDA),glutathione peroxidase(GSH-Px),peroxidase(CAT),mitochondrial function related indexes,mitochondrial respiratory chain complexⅠandⅢactivities,energy metabolism related indexes Na^(+)-K^(+)-ATPase,Ca^(2+)-ATPase activity and expression of hypoxia adaptation gene hypoxia inducible factor 1α(HIF-1α),erythropoietin(EPO)and vascular endothelial growth factor(VEGF)were measured.The results showed that compared with the control group,the treatment of different concentrations of CA significantly reduced ROS activity(P<0.05),significantly reduced the content of antioxidant index MDA(P<0.05),increased the activities of SOD,GSH-Px and CAT(P<0.05),and significantly increased the activities of mitochondrial respiratory chain complexⅠandⅢ(P<0.05),significantly increase the activities of Na^(+)-K^(+)-ATPase and Ca^(2+)-ATPase(P<0.05),significantly increase the mRNA and protein expression of HIF-1α,EPO and VEGF(P<0.05).The results between test groups showed that,in 40mg/kg group,the activity of ROS was significantly decreased(P<0.05),the content of MDA was significantly reduced(P<0.05),and the activity of SOD was significantly increased(P<0.05),the activities of GSH-Px and CAT were extremely significant increased(P<0.01).In 20mg/kg and 40mg/kg groups,the activity of respiratory chain complexⅢwas extremely significant increased(P<0.01),in 40mg/kg group,the activity of Na^(+)-K^(+)-ATPase was extremely significant increased(P<0.01)and the activity of Ca^(2+)-ATPase was significantly increased(P<0.05),the mRNA expression of HIF-1αand EPO were extremely significant increased(P<0.01),the protein expression of HIF-1αand EPO were significantly increased(P<0.05).In conclusion,CA can play a myocardial protective role by inhibiting ROS activity in myocardial tissue of SD rats,enhancing its antioxidant capacity,mitochondrial function,energy metabolism level and hypoxia adaptability,enhance hypoxia adaptability in myocardial tissue of SD rats,and 40mg/kg group has the best efficacy.
作者
刘嘉华
吴华
行倩文
刘波
张龙飞
王梦杰
陈鑫磊
王文胜
LIU Jiahua;WU Hua;XING Qianwen;LIU Bo;ZHANG Longfei;WANG Mengjie;CHEN Xinlei;WANG Wensheng(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2023年第2期348-356,共9页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(32160812)。
关键词
低氧
菊苣酸
心肌组织
低氧适应
hypoxia
cichoric acid
myocardial tissue
hypoxia adaptability
