摘要
为了确定肾茶叶枯病致病病原菌,笔者从肾茶云南产区采集的肾茶叶枯病样本中分离到1株病原菌,并对其进行了病害症状观察,病原菌分离、鉴定和病原菌生物学特性研究。结果表明,其在PDA培养基上菌落为白色,气生菌丝发达,菌落初期下部淡粉色,后期为深黄棕色,分生孢子顶胞钩状,成熟的大型分生孢子有3~5个隔膜。将病原菌离体接种到健康肾茶叶片,保湿培养数天后接种部位出现黑褐色病斑,与田间症状一致。病原菌基因组DNA经真菌rDNA-ITS通用引物ITS1/ITS4扩增及同源性分析,病原菌与Fusarium nematophilum,Fusarium equiseti,Fusarium chlamydosporum,Fusarium longipes聚为一支,核酸序列同源性为99.40%~99.60%。结合形态特征观察、ITS序列分析及柯赫氏法则验证结果,初步确定该病原菌为镰刀菌。
In order to determine the pathogen of leaf blight of kidney plant(Clerodendranthus spicatus(Thunb.)C.Y.Wu),the kidney plant leaves infected with leaf blight were sampled from a kidney plant producing area in Yunnan,and one strain of the pathogen was isolated from the samples and then identified morphologically and molecularly.The results showed that the pathogen cultured on the PDA medium was white in colony,and well developed in aerial mycelia.The colony in the lower part was pale pink initially and then turned yellow-brown.The apical cells of the conidia were uncinate,and the mature macroconidia have 3~5 septa.The pathogen isolated was inoculated onto the healthy plant leaves of C.spicatus.After several days of moist culture,dark-brown lesions appeared at the inoculated sites of the leaves,which were consistent with the field symptoms.Genomic DNA of the pathogen was amplified by using fungal rDNA-ITS universal primers ITS1/ITS4 and analyzed by homology analysis.The results showed that the pathogen isolated was clustered into the same branch with Fusarium nematophilum,F.equiseti,F.chlamydosporum and F.longip,and had a nucleic acid sequence homology of 99.40%−99.60%.The morphological observation,ITS sequence analysis and establishment of Koch’s postulates showed that the pathogen was preliminarily identified as F.oxysporum.
作者
商瑞
毛佳
王延谦
白亭亭
杨春勇
王红卫
王艳芳
郭睿
李戈
SHANG Rui;MAO Jia;WANG Yanqian;BAI Tingting;YANG Chunyong;WANG Hongwei;WANG Yanfang;GUO Rui;LI Ge(Yunnan Medical Health College,Kunming,Yunnan 650033;Agricultural Environment and Resources Institute,Yunnan Academy of Agricultural Sciences,Kunming,Yunnan 650205;Yunnan Branch of Research Institute of Medicinal Plants,Chinese Academy of Medical Sciences,Jinghong,Yunnan 666100,China)
出处
《热带生物学报》
2023年第2期229-233,共5页
Journal of Tropical Biology
基金
云南省教育厅科学研究基金项目(2021J1361)。
关键词
肾茶
叶枯病
病原分离
病原鉴定
Clerodendranthus spicatu
leaf blight
pathogen isolation
pathogen identification
