摘要
[目的]探究龙眼Switching 3(SWI3)、Actin-related protein(ARP)、SWR1 complex(SWC)和Bushy(BSH)亚基的潜在功能。[方法]利用生物信息学分析其分子进化特性,用转录组和实时荧光定量PCR(real-time quantitative PCR,q RT-PCR)分析基因在体胚发生早期胚性愈伤组织(embryogenic callus,EC)、不完全胚性紧实结构(incomplete embryogenic compact structures,ICpEC)和球形胚(globular embryos,GE)阶段及在EC中响应激素的表达模式。[结果]龙眼含4个DlS WI3、10个DlA RP、4个DlS WC和1个DlB SH蛋白,它们大多不稳定且主要定位在细胞核、细胞质和叶绿体上。DlS WI3和DlA RP家族扩张受基因复制驱动,而4个DlSWC和1个DlBSH基因由单拷贝产生。DlS WI3、DlA RP、DlS WC和DlB SH结构域各异,进化上与拟南芥、水稻和甜橙一致。启动子预测表明,DlSWI3、DlARP、DlSWC和DlBSH亚基的基因含多种激素、胁迫、光和生长发育相关元件。q RT-PCR分析显示,从EC分化到GE,DlARP4、DlARP5-3、DlARP9和DlBSH逐渐下调,而DlSWI3C、DlARP8和DlSWC2逐渐上调,且DlSWI3D在GE特异高表达;DlARP5-3、DlSWC2、DlSWI3D和DlBSH在脱落酸(abscisic acid,ABA)处理12 h时被显著上调,DlARP4、DlARP5-3、DlSWC2、DlSWI3D和DlBSH在茉莉酸甲酯(methyl jasmonate,MeJ A)处理4 h时被显著上调,5个基因被赤霉素(gibberellin A3,GA3)或水杨酸(salicylic acid,SA)下调,DlARP4被ABA下调。[结论]DlS WI3、DlA RP、DlS WC、DlB SH亚基在进化上高度保守,它们可能通过响应激素诱导影响染色质重塑活性,调控龙眼早期体胚发育。
[Objective]To understand the potential functions of Switching 3(SWI3),Actin-related protein(ARP),SWR1 Complex(SWC)and Bushy(BSH)subunits in longan.[Method]Using bioinformatics analyzed their molecular evolution characteristics,and the expression patterns of genes in embryogenic callus(EC),incomplete embryogenic compact structures(ICpEC)and globular embryos(GE)stages of early somatic embryogenesis,and that of genes respond to hormones in EC,were analyzed by using transcriptome and real-time quantitative PCR(qRT-PCR).[Result]Longan contains(4 DlSWI3,10 DlARP,4 DlSWC and 1 DlBSH)proteins,which are mostly unstable and mainly located in the nucleus,cytoplasm and chloroplast.Expansion of DlSWI3 and DlARP families is driven by gene duplication,while four DlSWC and one DlBSH genes are generated by single-copy events.DlSWI3,DlARP,DlSWC and DlBSH have different domains,which are evolutionarily consistent with Arabidopsis thaliana,Oryza sativa,and Citrus sinensis.Promoter predictions indicate that genes encoding DlSWI3,DlARP,DlSWC and DlBSH subunits contain a variety of cis-acting elements related to hormone,stress,light,growth and development.qRT-PCR analysis showed that DlARP4,DlARP5-3,DlARP9 and DlBSH were gradually down-regulated from EC differentiation to GE,while DlSWI3C,DlARP8 and DlSWC2 were gradually up-regulated,and DlSWI3D was highly expressed in GE;DlARP5-3,DlSWC2,DlSWI3D and DlBSH were significantly up regulated at 12 h of abscisic acid(ABA)treatment,and DlARP4,DlARP5-3,DlSWC2,DlSWI3D and DlBSH were significantly up regulated at 4 h of methyl jasmonate(MeJA)treatment,while five genes were down regulated by gibberellin A3(GA3)or Salicylic acid(SA)and DlARP4 was down regulated by ABA.[Conclusion]DlSWI3,DlARP,DlSWC,and DlBSH subunits are highly conserved in evolution,and they may influence chromatin remodeling activity in response to hormone induction to regulate early somatic embryo development in longan.
出处
《园艺与种苗》
CAS
2023年第2期53-59,共7页
Horticulture & Seed
基金
国家自然科学基金(No.31572088,No.31672127)
福建省高原学科建设经费项目(No.102/71201801101)
福建农林大学科技创新基金项目(No.CXZX2020027A)。
关键词
龙眼
体胚发生
激素
染色质重塑
生物信息学
Dimocarpus longan Lour.
Somatic embryogenesis
Hormone
Chromatin remodeling
Bioinformatics
