摘要
为对比分析辣椒不同栽培土壤/基质中真菌差异、揭示辣椒无土栽培基质中真菌群落结构特性,分别采集辣椒无土栽培根际基质(SlPlRh)、无土栽培非根际基质(SlPlNRh)、根际土壤(PlRh)、非根际土壤(PlNRh)以及非耕作土壤(NPl)作为试验素材。利用Illumina-Seq高通量测序技术检测5种土壤/基质中真菌多样性及丰度,分析真菌群落结构特征。结果表明:PlNRh真菌丰度和多样性最高,SlPlNRh最低,两者丰度存在显著差异,其他样品间丰度和多样性均无显著差异;真菌群落组成在门分类水平PlRh、SlPlRh和SlPlNRh的组成较相似,平均相对丰度占比有差异,属分类水平NPl、PlRh和PlNRh组成相似,SlPlRh和SlPlNRh组成相似;真菌群落聚类分析表明NPl、PlRh、PlNRh 3种土壤和SlPlRh、SlPlNRh 2种基质明显分成两个类群;LEfSe分析结果显示PlRh和PlNRh两种土壤的显著性差异物种最多、SlPlRh最少。通过检测辣椒种植土壤和无土栽培基质中真菌多样性及群落组成,比较分析不同类型种植基质根际真菌微生物组的差别,初步揭示辣椒无土栽培基质真菌多样性和群落结构特征,为今后无土栽培基质选择、基质组分配比和改良提供科学依据。
In order to compare and analyze the differences of fungi in different cultivated soils/substrates of pepper and reveal the characteristics of fungal community structure in the soilless culture substrates of pepper,the rhizosphere matrix(SlPlRh)for the soilless culture of pepper,non-rhizosphere matrix for the soilless culture(SlPlNRh),rhizosphere soil(PlRh),non-rhizosphere soil(PlNRh)and uncultivated soil(NPl)were collected as the experimental materials.Then,the diversity and abundance of fungi in the five kinds of soils/substrates were detected by using the Illumina Seq high-throughput sequencing technology,and the structural characteristics of fungal community were analyzed.The results showed that PlNRh had the highest fungal abundance and diversity,while SlPlNRh had the lowest.And there were significant differences in the abundance between the two samples,while there were no significant differences in the abundance and diversity among other samples.The fungal community composition of PlRh,SlPlRh and SlPlNRh was similar at the phylum classification level,and the average relative abundance ratio was different.At the genus classification level,the composition of NPl,PlRh and PlNRh was similar,and the composition of SlPlRh and SlPlNRh was similar.The cluster analysis of fungal community showed that the three kinds of soil(NPl,PlRh and PlNRh)and the two kinds of substrates(SlPlRh and SlPlNRh)were obviously divided into two class groups.The lEfSe analysis showed that PlRh and PlNRh had the most species with significant difference,while SlPlRh had the fewest.By detecting the diversity and community composition of fungi in the planting soil and the soilless culture substrates of pepper,the differences of the rhizosphere fungal microbiome in different types of planting substrates were compared and analyzed.Then,the diversity and community structure characteristics of fungi in the soilless culture substrates of pepper were preliminarily revealed,which provided scientific basis for the selection of soilless culture substrates,the distribution ratio and improvement of matrix group in the future.
作者
马慧斐
朱海生
李永平
黄昊
康玉妹
温庆放
薛珠政
MA Hui-fei;ZHU Hai-sheng;LI Yong-ping;HUANG Hao;KANG Yu-mei;WEN Qing-fang;XUE Zhu-zheng(Fujian Key Laboratory of Vegetable Genetic Breeding/Crop Research Institute,Fujian Academy of Agricultural Sciences/Fujian Research Center of Vegetable Engineering Technology,Fuzhou,Fujian 350013,China)
出处
《福建农业科技》
CAS
2023年第1期43-50,共8页
Fujian Agricultural Science and Technology
基金
福建省农业科学院自由探索项目(ZYTS2019008)
国家特色蔬菜产业技术体系福州综合试验站项目(CARS-24-G-08)。
关键词
辣椒
无土栽培
基质
真菌群落结构
多样性
Pepper
Soilless culture
Substrate
Fungal community structure
Diversity
