摘要
1-氨基环丙烷-1-羧酸合成酶(1-aminocyclopropane-1-carboxylic acid synthase,ACS)作为乙烯生物合成途径的限速酶,由多基因家族编码,对于乙烯在高等植物体内调控代谢和生长发育具有重要的意义。本研究利用生物信息学方法鉴定得到13个黄梁木ACS基因,并对其编码的蛋白进行系统进化和表达模式分析。结果显示,Nc ACSs可分为Ⅰ型、Ⅱ型、Ⅲ型。序列分析显示,NcACSs基因长度在1412~4257 bp之间,含有3~5个外显子,共分布在10条染色体上。NcACS蛋白序列长度为327~537 aa,NcACS蛋白有7个显酸性和6个显碱性,且均为亲水性蛋白,其中包含3个稳定蛋白。NcACS蛋白具有多个潜在磷酸化位点,可通过磷酸化位点调节ACS酶活性。ACSs系统进化分析显示,NcACS6.1和NcACS6.2与AtACS6亲缘关系较近,推测NcACS6.1和Nc ACS6.2在功能上与AtACS6相似,在响应非生物胁迫时具有重要意义。通过转录组测序技术,对Nc ACSs进行表达模式分析,结果表明,NcACSs基因具有时空特异性,其中NcACS1、NcACS6.2和NcACS10.3在形成层中表达量相对较高,可能参与黄梁木次生生长中的木质部扩增。本研究为深入研究黄梁木NcACSs基因的功能以及探索ACS酶对速生林木生长发育的影响提供理论基础。
ACS(1-aminocyclopropane 1-carboxylic acid synthase)as a speed limit enzyme ofethylene biosynthesis pathway,are encoded by multi-gene families,for ethylene regulation of metabolism in higher plant body and is of great importance to the growth and development.This study using bioinformatics methods to identify 13 A CS genes in Neolamarckia cadamba,for its protein system evolution analysis,according to the results of NcACSs can be divided into typeⅠ,typeⅡ,typeⅢ.Sequence analysis showed that the length of NcA CS genes ranged from 1412 bp to 4257 bp,containing 3 to 5 exons,and the proteins ranged from 327 to 537 aa,located on 10 chromosomes.Among the NcACS proteins,7 proteins were acidic and 6 proteins were alkaline,and all of them were hydrophilic proteins,including 3 stable proteins.NcACS proteins have multiple potential phosphorylation sites,through which ACS enzyme activity can be regulated.The phylogenetic analysis showed that,NCACS6.1 and NCACS6.2 were closely related to AtACS6,so it was speculated that NcACS6.1 and NcACS6.2 were functionally similar to AtACS6 and had important significance in response to abiotic stress.Through the RNA-seq technology,NcACSs expression pattern analysis,the results showed that the NcACS genes specificity of time and space,can blossom in growth,development,ripe and play different roles in different periods,including the expression of NcACS1,NcACS6.2 and NcACS10.3 is relatively high in the cambium,may participate in xylem amplification of Neolamarckia cadamba secondary growth.This study provides a theoretical basis for further research on the function of NcACS genes in Neolamarckia cadamba and the effects of ACS enzymes on the growth and development of fast-growing trees.
作者
高佳钰
游晓商
张瞳
彭昌操
Gao Jiayu;You Xiaoshang;Zhang Tong;Peng Changcao(College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou,510642)
出处
《分子植物育种》
CAS
北大核心
2023年第7期2200-2210,共11页
Molecular Plant Breeding
基金
广东省林业科技创新重点项目(2016KJCX003,2019KJCX001)
国家重点研发计划项目(2016YFD0600104)
国家自然科学基金项目(30771759,31170636,31470681)
广东省自然科学基金项目(2016A030311032)
广州市科技计划项目(201607020024)
广东省高等教育青年创新人才基金(2017KQNCX017)共同资助。
