摘要
低温是限制苜蓿生长和产量的主要环境因素。黄花苜蓿抗低温能力强,是苜蓿育种中重要的抗性基因源。JAZ蛋白(Jasmonate ZIM Domain)作为茉莉素调控途径中的重要负调控因子,是消减茉莉素信号,实现作物生长与防御之间平衡的调节器。因此了解黄花苜蓿的抗低温能力具有重要的科学和生产意义。为此,本研究以呼伦贝尔黄花苜蓿(Medicago falcata cv.Hulunbeier)为试验材料,采用实时荧光定量PCR技术分析了转录组筛选出的15个JAZ基因在低温胁迫黄花苜蓿地上和地下组织中的表达模式。结果显示,这15个JAZ基因主要在黄花苜蓿地上组织中表达,在地上组织和地下组织中受低温胁迫12 h内上调表达,表明它们在调节黄花苜蓿低温耐受性的茉莉素信号中起着重要作用。研究结果有助于黄花苜蓿的抗低温候选基因的筛选,并为其研究耐低温分子机制提供依据。
Low temperature is the main environmental factor limiting the growth and yield of alfalfa.Medicago falcata is an important source of resistance genes in alfalfa breeding for its resistance to low temperature stress.JAZ(Jasmonate ZIM Domain),an important negative regulatory factor in the jasmonate pathway,is the regulator of reducing jasmonate signal and achieving the balance between crop growth and defense.Therefore,it is of great sci-entific and production significance to understand the low temperature resistance ability of M.falcata.In this study,we used M.falcata cv.Hulunbeier as experimental material,and analyzed the expression patterns of 15 JAZ genes screened out by transcriptome in the above-ground and underground tissues of M.falcata under low temperature stress by real-time fluorescence quantitative PCR.We found that the 15 JAZ genes were mainly expressed in shoots of M.falcata,and up-regulated in shoots and roots after 12 h of low temperature stress.These results indi-cating that these genes played an important role in the regulation of jasmonate signaling of low temperature toler-ance of M.falcata.These results will be helpful for screening candidate genes of low temperature tolerance in M.falcata and layed a foundation for studying the molecular mechanism of low temperature tolerance in M.falcata.
作者
杨梅
臧军蕊
王召誉
段新航
刘家言
刘学生
肖汇川
魏亚娇
张攀
Yang Mei;Zang Junrui;Wang Zhaoyu;Duan Xinhang;Liu Jiayan;Liu Xuesheng;Xiao Huichuan;Wei Yajiao;Zhang Pan(College of Animal Science and Technology,Northeast Agricultural University,Harbin,150030)
出处
《分子植物育种》
CAS
北大核心
2023年第7期2211-2219,共9页
Molecular Plant Breeding
基金
国家自然科学基金项目(31502007)
哈尔滨市科技局科技创新人才项目(2017RAQXJ008)共同资助。
关键词
黄花苜蓿
低温胁迫
表达模式分析
实时荧光定量PCR
Medicago falcata L.
Low-temperature stress
Expression pattern analysis
Quantitative real-time PCR
