摘要
食品检测标准物质是保证食品检测量值溯源性、互认性和准确性的测量标准,其研制需要精准的定值方法。数字PCR法是灵敏、准确和抗基质干扰强的核酸绝对定量方法,已在医疗诊断、转基因检测和动物疫病检测等众多领域广泛应用。对数字PCR法进行介绍,综述其在转基因食品检测、动植物源性分析和食源性致病菌检测标准物质研制中的研究进展,以期为食品检测核酸标准物质的研制提供参考。
Food detection reference material is a measuring instrument to ensure traceability,mutual recognition and accuracy of food detection value,and its development needs accurate determination methods.Digital PCR is a sensitive,accurate and matrix-resistant method for absolute quantification of nucleic acid,which is widely applied in many fields such as medical diagnosis,genetically modified organism detection and animal disease detection.Digital PCR method and its research progress in the detection of genetically modified foods,animal and plant origin analysis and the development of food-borne pathogenic bacteria detection reference materials is reviewed to provide reference for the development of food detection nucleic acid reference materials.
作者
范宏博
蔡永洪
李月玥
黄满英
张力玲
马婷婷
胡松青
胡良勇
FAN Hong-bo;CAI Yong-hong;LI Yue-yue;HUANG Man-ying;ZHANG Li-ling;MA Ting-ting;HU Song-qing;HU Liang-yong(Guangzhou Institute of Measurement and Testing Technology,Guangzhou,Guangdong 510700,China;School of Food Science and Engineering,South China University of Technology,Guangzhou,Guangdong 510630,China)
出处
《计量学报》
CSCD
北大核心
2023年第3期472-478,共7页
Acta Metrologica Sinica
基金
广东省重点领域研发计划项目(2020B0404010002)。
