摘要
[目的]揭示杜仲种子内的微生物群落组成和多样性,丰富杜仲相关微生物资源。[方法 ]本研究采用传统培养法和高通量测序技术对杜仲种子内生微生物群落组成和多样性进行分析,同时基于FAPROTAX和FUNGuild数据库对其进行功能注释。[结果 ]从杜仲种子中共分离获得真菌40株(3门11属),优势属为曲霉属(Aspergillus)(30%);细菌142株(1门6属),其中,土壤芽孢杆菌属(Solibacillus)(47.18%)为主要优势属。高通量测序获得141个真菌OTUs,隶属于6门101属;442个细菌OTUs,隶属于24门313属。在属水平,种子优势真菌属为Apiotrichum(31.28%)和德巴利氏酵母属(Debaryomyces)(26.07%);优势细菌属主要为假单胞菌属(Pseudomonas)(16.66%)和乳杆菌属(Lactobacillus)(9.68%)。功能注释结果显示:真菌以降解有机物、促进宿主植物生长、促进宿主对土壤养分的吸收、改善土壤环境微生物群落结构以及致病菌群为主;细菌以化能异氧、有氧化能异养以及具有发酵和固氮作用的菌群为主。[结论 ]杜仲种子内携带多种有益功能菌和潜在致病菌,这些微生物对植物的生长发育具有重要影响。同时本研究还分离到多株有益功能菌,这将为后续人为调控种子微生物组以增强植物健康提供微生物资源。
[Objective]To reveal the microbial community composition and diversity in Eucommia ulmoides seeds and enrich the related microbial resources.[Methods]In this study,the diversity of endophytic microorganisms in E.ulmoides seeds was analyzed by traditional culture method and high-throughput sequencing technology,and functional annotated by FAPROTAX and FUNGuild database.[Results]A total of 40 fungi strains(3 phyla and 11 genera)were isolated from E.ulmoides seeds,and the dominant genus was Aspergillus(30%);142 bacterial strains(1 phylum,6 genera)were isolated,of which Solibacillus was the dominant genus(47.18%).Through high-throughput sequencing,141 fungal OTUs were obtained belonging to 6 phylum and 101 genera;442 bacterial OTUs were obtained belonging to 24 phylum and 313 genera.At the genus level,the dominant fungi were Apiotrichum(31.28%)and Debaryomyces(26.07%);The dominant bacterial genera were Pseudomonas(16.66%)and Lactobacillus(9.68%).The results of functional annotation showed that fungi mainly degraded organic matter,promoted the growth of host plants and the absorption of soil nutrients by host,improved soil microbial community structure and Pathogen community.The bacteria mainly consisted of chemoheterotrophic,heterotrophic,fermentation.and nitrogen fixation bacteria.[Conclusion]E.ulmoides seeds carry a variety of probiotics and potential phytopathogens,which have important effects on plant growth and development.In addition,a number of beneficial functional bacteria were isolated in this study,which will provide microbial resources for the subsequent artificial control of seed microbiome to enhance plant health.
作者
张青青
董醇波
邵秋雨
陆莹霞
董旋
梁宗琦
韩燕峰
ZHANG Qing-qing;DONG Chun-bo;SHAO Qiu-yu;LU Ying-xia;DONG Xuan;LIANG Zong-qi;HAN Yan-feng(Institute of Fungus Resources,Department of Ecology,College of Life Sciences,Guizhou University,Guiyang 550025,Guizhou,China;College of Tea Science,Guizhou University,Guizhou University,Guiyang 550025,Guizhou,China)
出处
《林业科学研究》
CSCD
北大核心
2023年第2期50-60,共11页
Forest Research
基金
国家自然科学基金(32260003,32060011)
贵州省“百层次”创新型人才培养项目([2020]6005)
贵州省生物学一流学科建设项目(GNYL[2017]009)。
关键词
高通量测序
可培养法
多样性
生态功能
high-throughput sequencing
culturable method
diversity
ecological function
