摘要
莲子心作为一种药食同源的植物,极具开发潜力。该研究通过单因素实验和响应面Box-Behnken设计实验筛选并优化莲子心总黄酮的多元醇超声辅助提取工艺,对该工艺获得的莲子心提取物进行了安全性及体外抗氧化、抑菌和抗敏活性的测定。结果表明,在提取溶剂为1,2-己二醇,溶剂体积分数87%,料液比3.6∶100(g∶mL),提取时间39 min的条件下,莲子心总黄酮得率为1.80%±0.08%,且其质量浓度在1.2 g/L以下时无刺激性,对DPPH和ABTS自由基的半数抑制质量浓度分别为0.76和0.69 g/L,对大肠杆菌、金黄色葡萄球菌和铜绿假单胞菌的最小抑菌质量浓度分别为0.56,1.12和0.14 g/L;且其对透明质酸酶的半数抑制质量浓度为0.35 g/L。该研究提供了一种简单、安全、高效的莲子心总黄酮提取工艺,在保证较高总黄酮得率的基础上兼顾其提取物的生物活性,为莲子心资源的开发提供了理论依据。
Nelumbinis Plumula is a kind of medicinal and edible plant with great development potential.Single factor test and response surface test were used to screen and optimize the ultrasonic-assisted extraction process of total flavonoids from Nelumbinis Plumula by polyols,and the safety,in vitro antioxidant,antibacterial and anti-allergy activities of Nelumbinis Plumula extract were determined.Results show that under the conditions of extraction solvent 1,2-hexanediol,solvent volume fraction 87%,material-liquid ratio 3.6∶100(g∶mL),and extraction time 39 min,the yield of total flavonoids is 1.80%±0.08%.At 1.2 g/L,the Nelumbinis Plumula extract has no irritation,the half maximal inhibitory concentration of DPPH radical and ABTS radical is 0.76 and 0.69 g/L,respectively,and the minimum inhibitory concentration for Escherichia coli,Staphylococcus aureus and Pseudomonas aeruginosa are 0.56,1.12 and 0.14 g/L,respectively.Moreover,the half maximal inhibitory concentration of hyaluronidase is 0.35 g/L.This study provides an easy,safe and efficient method for extracting total flavonoids from Nelumbinis Plumula,which ensures the biological activity of the extract and a high yield of total flavonoids,and provides a theoretical basis for the development of Nelumbinis Plumula.
作者
郝晴
吴二敏
毕永贤
刘金俊
孔德承
成志伟
Qing Hao;Ermin Wu;Yongxian Bi;Jinjun Liu;Decheng Kong;Zhiwei Cheng(Key Laboratory of Plant Resources Research and Development,College of Chemistry and Materials Engineering,Beijing Technology and Business University,Beijing 100048,China;Zhejiang Yige Enterprise Management Group Co.,Ltd,Hangzhou,Zhengjiang 310000,China)
出处
《日用化学工业（中英文）》
CAS
北大核心
2023年第4期398-407,共10页
China Surfactant Detergent & Cosmetics
基金
北京教委科技面上项目(SQKM201710011010)。
关键词
莲子心
响应面
总黄酮
抗氧化活性
抑菌活性
抗敏活性
Nelumbinis Plumula
response surface
total flavonoids
antioxidant activity
antibacterial activity
antiallergy activity
