摘要
为预测高产吩嗪类物质铜绿假单胞菌(Pseudomonas aeruginosa,PA)工程菌株PA2019NES在农用开发时的生物安全性,本文采用实时荧光定量PCR技术,研究多拷贝phzR基因的PA2019NES与PA2016NX1在生物被膜状态(被膜态)、浮游状态(浮游态)的生物被膜、群体感应系统、六型分泌系统共9个基因转录量差异,结果显示:在浮游态下,多拷贝phzR基因上调vgrG、algD基因的mRNA转录量(P<0.05),下调pilA基因mRNA合成(P<0.05),但其他6个基因的转录量无显著差异(P>0.05);在被膜态下,仅提高rhlI基因的mRNA表达量(P<0.05),而fimX和wspR基因的mRNA合成受到抑制(P<0.05),但其他6个基因的转录量无显著影响(P>0.05);经24 h培养后,PA2016NX1和PA2019NES的蹭行运动无显著差异(P>0.05),但游泳运动后者强于前者(P<0.05),集群运动后者弱于前者(P<0.05)。此结果证明PA2019NES的条件致病性能可能不低于PA2016NX1,在农用开发该菌株时应杜绝活菌向环境扩散。
In order to prognosis the biosecurity of PA2019NES which is with high yield of phenazines in agricultural exploitation,the real-time fluorescence quantitative PCR was applied to test the difference of transcriptional volume of 9 genes which were associated with biofilm,quorum sensing system and typeⅥsecretory system,to reveal the transcriptional relationship between phzR and the above mentioned 9 genes,as well as its effect on the cells motility,especially the differeces between PA2016NX1 and PA2019NES,which was with multiple copies of phzR gene,were in biofilm and planktonic state,respectively.The results showed that,in the planktonic state,multiple copies phzR gene up-regulated the mRNA level of vgrG and algD genes(P<0.05),the mRNA level of pilA was down-regulated(P<0.05);however,the mRNA level of the other 6 genes was not significantly affected(P>0.05).In the biofilm state,the mRNA level of rhlI gene was up-regulated(P<0.05),but that of fimX and wspR genes was down-regulated(P<0.05)and that of the other 6 genes was not significantly affected(P>0.05).After 24 h culture,there was no significant difference in twitching ability between PA2016NX1 and PA2019NES(P>0.05),but the swimming,ability of PA2019NES was stronger than that of PA2016NX1(P<0.05),however,the swarming ability of PA2019NES was weaker than that of PA2016NX1(P<0.05).These results suggested that the power of conditional pathogenicity of PA2019NES may not be lower than that of PA2016NX1,hence,when PA2019NES was developed for agricultural use,the spread of live bacteria to the environment should be prevented.
作者
戴明瑶
李亚诗
黄新妮
肖君
黄植清
吕春萌
陆祖军
DAI Mingyao;LI Yashi;HUANG Xinni;XIAO Jun;HUANG Zhiqing;LÜChunmeng;LU Zujun(College of Life Science,Guangxi Normal University,Guilin Guangxi 541006,China;Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection(Guangxi Normal University),Ministry of Education,Guilin Guangxi 541006,China)
出处
《广西师范大学学报（自然科学版）》
CAS
北大核心
2023年第2期161-174,共14页
Journal of Guangxi Normal University:Natural Science Edition
基金
国家自然科学基金(31860010)
广西大学生创新创业训练项目(201910602061,202010602055,202010602256)。
