摘要
本研究采用薄膜水合法制备表没食子儿茶素没食子酸酯(EGCG)/姜黄素(Cur)复合脂质体(EGCG/Cur-L),通过单因素实验确定最佳制备工艺。为了增加脂质体的稳定性,对EGCG/Cur-L表面进行二硬脂酰基磷脂酰乙醇胺-聚乙二醇2000(DSPE-PEG_(2000))修饰,并对两种脂质体的包封率、粒径分布、微观形态和修饰效果进行研究,同时采用DPPH法评价脂质体的氧化稳定性。结果表明:EGCG/Cur-L的最佳工艺为:卵磷脂与胆固醇质量比为6:1,PBS缓冲溶液pH为6.5,EGCG添加量为6 mg,Cur添加量为3 mg,水化温度为55℃。EGCG包封率为68.78%,Cur包封率为90.23%,平均粒径为183.8±5.4 nm,多分散系数(PDI)为0.178±0.01,平均Zeta-电位为-34.7±0.62 mV。修饰后EGCG包封率为60.31%,Cur包封率为88.53%。表面修饰后Cur的包封率无明显变化,EGCG包封率有所下降;动态光散射(DLS)和透射电子显微镜(TEM)结果表明,修饰后脂质体的平均粒径从未修饰的183.8 nm增大到374.5 nm;Zeta-电位和傅里叶红外分析结果表明DSPE-PEG_(2000)成功修饰在脂质体表面,且不改变脂质体内部结构。修饰脂质体DPPH清除率最高,在15 d内氧化稳定性均大于其他脂质体,表明DSPE-PEG_(2000)可以增强脂质体的抗氧化能力。
In this study,epigallocatechin gallate(EGCG)/curcumin(Cur)composite liposomes(EGCG/Cur-L)were prepared by thin-film hydrophoresis,and the optimal preparation process was determined by single-factor test.To increase the stability of liposomes,the surface of the composite liposomes was modified with distearoyl phosphatidylethanolamine-polyethylene glycol 2000(DSPE-PEG_(2000)),and the encapsulation rate,particle size distribution,micromorphology,and modification effect of both liposomes were investigated,and the oxidative stability of liposomes was evaluated by DPPH method.The results showed that the optimal process for the composite liposomes was:Lecithin to cholesterol mass ratio of 6:1,PBS buffer solution pH of 6.5,EGCG added amount 6 mg,Cur added amount 3 mg,and hydration temperature of 55℃.The encapsulation rate of EGCG was 68.78%,the encapsulation rate of Cur was 90.23%,the average particle size was 183.8±5.4 nm,the polydispersity coefficient(PDI)was 0.178±0.01,and the average Zeta-potential was−34.7±0.62 mV.The encapsulation rate of EGCG after modification was 60.31%,and the encapsulation rate of Cur was 88.53%.After surface modification,the encapsulation rate of Cur did not change significantly,and the encapsulation rate of EGCG decreased,dynamic light scattering(DLS)and transmission electron microscopy(TEM)results showed that the average particle size of the modified liposomes increased from the unmodified 183.8 nm to 374.5 nm,Zeta-potential and Fourier infrared analysis indicated that DSPE-PEG_(2000)was successfully modified on the liposome surface and did not change the internal structure of the liposomes.The modified liposomes had the highest DPPH scavenging rate and all had greater oxidative stability than other liposomes within 15 days,indicating that DSPE-PEG_(2000)could enhance the antioxidant capacity of liposomes.
作者
李亮
袁传勋
王敏
朱梦男
金日生
LI Liang;YUAN Chuanxun;WANG Min;ZHU Mengnan;JIN Risheng(School of Food and Bioengineering,Hefei University of Technology,Hefei 230601,China;Engineering Center of Agricultural Products Biochemical Engineering,Ministry of Education,Hefei 230601,China)
出处
《食品工业科技》
CAS
北大核心
2023年第9期88-95,共8页
Science and Technology of Food Industry
基金
安徽省科技重大专项(202003a06020011)。
