摘要
Dietary flavonoids are abundant in natural plants and possess multiple pharmacological and nutritional activities.In this study,apigenin,luteolin,and baicalein were chosen to evaluate their anti-diabetic effect in high-glucose and dexamethasone induced insulin-resistant(IR)HepG2 cells.All flavonoids improves the glucose consumption and glycogen synthesis abilities in IR-HepG2 cells via activating glucose transporter protein 4(GLUT4)and phosphor-glycogen synthase kinase(GSK-3β).These fl avonoids signifi cantly inhibited the production of reactive oxygen species(ROS)and advanced glycation end-products(AGEs),which were closely related to the suppression of the phosphorylation form of NF-κB and P65.The expression levels of insulin receptor substrate-1(IRS-1),insulin receptor substrate-2(IRS-2)and phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)pathway in IR-HepG2 cells were all partially activated by the fl avonoids,with variable effects.Furthermore,the intracellular metabolic conditions of the fl avonoids were also evaluated.
基金
supported by National Natural Science Foundation of China(32072212)
Multi-Year Research Grant of University of Macao(MYRG2018-00169-ICMS)
Science and Technology Development Fund of Macao(FDCT)(0098/2020/A)
MICINN supporting the Ramón y Cajal grant for M.A.Prieto(RYC-201722891)
Jianbo Xiao(RYC2020-030365-I)
Xunta de Galicia supporting the Axudas Conecta Peme,the IN852A 2018/58 Neuro Food Project,the program EXCELENCIA-ED431F 2020/12
the pre-doctoral grants of P.García-Oliveira(ED481A-2019/295)
to Ibero-American Program on Science and Technology(CYTED-AQUA-CIBUS,P317RT0003).
