摘要
The current study investigated a triad,which comprises of adipose tissue derived stem cells isolated from infrapatellar fat pad and gelatin/polyvinyl alcohol(PVA)-based matrix with exclusive ascorbic acid signalling.Though,the bio-mechanical properties of the gelatin–PVA blended scaffolds in wet condition are equivalent to the ECM of soft tissues in general,in this study,the triad was tested as a model for neural tissue engineering.Apart from being cytocompatible and biocompatible,the porosity of the scaffold has been designed in such a manner that it facilitates the cell signalling and enables the exchange of nutrients and gases.The highly proliferative stem cells from Passage 2 were characterized using both,mesenchymal and embryonic stem cell markers.As an initial exploration the mesenchymal stem cells at Passage 4 were exposed to ascorbic acid and basic fibroblast growth factor signalling for neuronal differentiation in 2D environment independently.The MSCs successfully differentiated and acquired neuron specific markers related to cytoskeleton and synapses.Subsequently,three phases of experiments have been conducted on the 3D gelatin/PVA matrix to prove their efficacy,the growth of stem cells,growth of differentiated neurons and the in situ growth and differentiation of MSCs.The scaffold was conducive and directed MSCs to neuronal lineage under specific signalling.Overall,this organotypic model triad could open a new avenue in the field of soft tissue engineering as a simple and effective tissue construct.
基金
National Foundation for Liver Research(NFLR)for financially supporting the cell-based work in this project.CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008–2011,Iniciativa Ingenio 2010,Consolider Program.CIBER actions are financed by the Instituto de Salud CarlosⅢwith assistance from the European Regional Development Fund
the University Grants Commission,India for the award of the Basic Scientific Research(UGC-BSR)Faculty Fellowship[No.F.4-5(11)2019(BSR)].
