摘要
目的:探讨祛瘀化痰二仙汤对老龄高尿酸血症大鼠阴茎勃起功能保护作用的可能机制。方法:将75只18月龄雄性SD大鼠分为正常组(N组)、模型组(M组)、祛瘀化痰二仙汤高剂量组、中剂量组、低剂量组(Qh组、Qm组、Ql组),每组15只。除正常组外,其余各组大鼠均建立高尿酸血症大鼠模型,并采用阿扑吗啡(APO)实验筛选勃起功能障碍(ED)大鼠。N组和M组均给予蒸馏水[10 ml/(kg·d)],Qh组[1.296 g/(kg·d)]、Qm组[0.648 g/(kg·d)]、Ql组[0.324 g/(kg·d)],每天给予药液灌胃1次,共12周。腹腔注射麻醉,检测大鼠阴茎海绵体阴茎海绵体内压(ICP)/平均动脉压(MAP)水平;处死大鼠后酶联免疫吸附法(ELISA)检测阴茎海绵体组织匀浆中一氧化氮(NO)、内皮素-1(ET-1)、白介素-1β(IL-1β)、白介素-18(IL-18)含量,蛋白免疫印迹法检测大鼠阴茎海绵体组织匀浆中核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)、凋亡相关斑点样蛋白(ASC)、半胱氨酸蛋白酶-1(Caspase-1)蛋白含量。结果:与N组比较,M组大鼠阴茎海绵体组织NO、ICP/MAP水平显著降低(P<0.05),而尿酸(UA)、NLRP3、ASC、Caspase-1、ET-1、IL-1β、IL-18水平均显著升高(P<0.05);与M组比较,Qh组阴茎海绵体组织NO、ICP/MAP水平显著升高(P<0.05),而UA、NLRP3、ASC、Caspase-1、ET-1、IL-1β、IL-18水平均显著降低(P<0.05)。结论:祛瘀化痰二仙汤可有效减轻高尿酸血液ED大鼠阴茎海绵体血管内皮功能损伤,改善勃起功能,其原因可能与其抑制阴茎海绵体组织NLRP3炎症小体介导的炎症反应有关。
Objective:To explore the possible mechanism of the protective effect of Quyuhuatanerxian decoction on erectile function in aged hyperuricemia rats.Methods:Seventy-five 18-month-old male SD rats were screened,were divided into normal group(N group),model group(M group),and Quyuhuatanerxian decoction high-dose group(Qh group),medium-dose group(Qm group),low-dose group(Ql group),with a total of 5 groups of 15 rats each.Except for the normal group,high uric acid ED model was established in all the groups.Erectile dysfunction(ED)rats were screened by apomorphine(APO)test.After successful modeling,distilled water was given in groups N group and M group[10 ml/(kg·d)];Qh group[1.296 g/(kg·d)],Qm group[0.648 g/(kg·d)],and Ql group[0.324 g/(kg·d)].The liquid was given intragastric once a day for 12 weeks.The rats were anesthetized by intraperitoneal injection,and the intracranial pressure(ICP)/mean arterial pressure(MAP)levels of the rat penis sponge were measured.The contents of nitric oxide(NO),endothelin-1(ET-1),interleukin-1β(IL-1β)and interleukin-18(IL-18)in the homogenate of cavernous tissue of the rats were determined by enzyme-linked immunosorbent assay(ELISA).The contents of nucleotide binding oligomeric domain-likereceptor protein 3(NLRP3),apoptosis-associated speck like protein(ASC)and Cysteine proteinase-1(Caspase-1)protein in the homogenate of rat cavernous tissue were determined by western blotting.Results:Compared with the N group,the expression levels of NO and ICP/MAP in penile corpus cavernosum tissues of rats in the M group were significantly decreased(P<0.05),while the expression levels of uric acid(UA),NLRP3,ASC,caspase-1,ET-1,IL-1a,IL-18,were significantly increased(P<0.05);Compared with the M group,the expression levels of NO and ICP/MAP in the corpus cavernosum of penis of Qh group were significantly increased(P<0.05),while the expression levels of UA,NLRP3,ASC,Caspase-1,ET-1,IL-1β,and IL-18 were significantly decreased(P<0.05).Conclusion:Quyuhuatanerxian decoction can effectively reduce vascular endothelial function impairment and improve erectile function in the penile corpus cavernosum of rats with hyperuricemic ED,which may be related to its inhibition of NLRP3 inflammasome mediated inflammatory response in the cavernous tissue of penis.
作者
谢红
金航
陈兰
陈智超
葛平玉
杨闽能
XIE Hong;JING Hang;CHEN Lan;CHEN Zhi-chao;GE Ping-yu;YANG Min-neng(Guizhou University of Traditional Chinese Medicine,Guiyang550025;The First Affiliated Hospital of Guizhou University of Chinese Medicine,Guiyang550001;Dejiang County National Hospital of Traditional Chinese Medicine of Guizhou Province,Dejiang565200)
出处
《实用中西医结合临床》
2023年第3期1-5,共5页
Practical Clinical Journal of Integrated Traditional Chinese and Western Medicine
基金
贵州省科学技术厅基础研究计划项目[编号:黔科合基础-ZK(2021)一般507]。
