摘要
为探究不同施肥处理对土壤类过氧化物酶活性的影响,以灰漠土和红壤长期定位试验站施肥处理土壤为研究对象,借助纳米矿物颗粒的类酶颜色反应原理,研究了不施肥(CK)、施用化肥(NPK)、施用化肥和有机肥(NPKM)和施用有机肥(M)土壤类过氧化物酶活性的变化。结果表明:与天然辣根过氧化物酶(HRP)相似,土壤也具有经典的催化颜色反应,灰漠土和红壤类过氧化物酶活性范围为0.4~1.0 U/kg。长期施肥显著改变了土壤类过氧化物酶活性,其中以M处理土壤类过氧化物酶活性为最高,可达0.7 U/kg,比CK处理增加了70%以上。不同施肥年限下,CK和NPK处理土壤类过氧化物酶活性并无显著差异,而M和NPKM处理土壤类过氧化物酶活性则随施肥时间线性增加(R^(2)=0.71,P<0.01)。高温灭菌处理后,土壤类过氧化物酶活性大多没有改变,表明其活性是土壤本身固有的氧化还原特性;但M处理红壤类过氧化物酶活性比CK和NPK处理红壤稳定性更高。皮尔森相关性分析表明,类过氧化物酶活性可能影响土壤可溶性矿质元素(如硅和镁)水平。因此,施肥措施可以通过改变土壤固有类过氧化物酶活性而影响养分周转。
To explore the effects of long-term fertilization on soil peroxidase(POD)-like activity,desert soil and red soil were collected from the different fertilization in two long-term fertilization stations aiming to compare their POD-like activities using the colorimetric reaction of nanozymes.The results show that soils exhibit a classic catalytic activity to natural horseradish peroxidase(HRP).POD-like activity of desert soil and red soil varies in a range of 0.4-1.0 U/kg.Long-term fertilization significantly alters soil POD-like activity.Among them,soils in the long-term manure(M)fertilization have the highest of POD-like activity(up to 0.7 U/kg),which is 70%higher than unfertilized soils.The fertilization time has no significant effects on soil POD-like activity from long term no fertilization(CK)and chemical fertilization(NPK).Notably,there is a linear correlation(R^(2)=0.71,P<0.01)between fertilization time and soil POD-like activity from long term organic fertilization(M and NPKM).High temperature sterilization alters soil POD-like activity,which is dependent on soil type and fertilization treatment.POD-like activity of red soil is relatively stable and not easily affected by environmental conditions,suggesting soil intrinsic POD-like activity rather than the absorption of extracellular enzymes on soils.In contrast,POD-like activities of soils in the NPK and CK treatments have a poorly stability and are easily affected by external environments.Pearson correlation indicates that soil POD-like activity is significantly correlated with dissolved silicon and magnesium.In conclusion,fertilization practices can alter soil intrinsic POD-like activity and then affect soil nutrient turnover.
作者
王苗苗
李波
余光辉
WANG Miaomiao;LI Bo;YU Guanghui(Institute of Surface-earth System Science,School of Earth System Science,Tianjin Key Laboratory of Earth Critical Zone Science and Sustainable Development in Bohai Rim,Critical Zone Observatory of Bohai Coastal Region,Tianjin University,Tianjin 300072,China;Shenergy Environmental Technology Co.ltd.,Hangzhou 311100,China)
出处
《土壤》
CAS
CSCD
北大核心
2023年第1期104-110,共7页
Soils
基金
国家自然科学基金项目(U22A20608,41977271)资助。
关键词
长期施肥
纳米酶
过氧化物酶
类芬顿反应
养分循环
Long-term fertilization
Nanozyme
Peroxidase
Fenton-like reaction
Nutrient cycling
