摘要
目的分析子痫前期患者胎盘环状RNA(circRNA)表达谱基因表达情况及其与患者病情的相关性。方法选取子痫前期患者(子痫前期组)15例,重度子痫前期患者(重度子痫前期组)25例及正常妊娠女性(对照组)30例。收集患者临床资料,检测各组24 h尿蛋白定量;采用酶联免疫吸附试验检测血清中的STOX1蛋白、血清可溶性血管内皮生长因子受体1(sFlt-1)、胎盘生长因子(PLGF)及血管内皮生长因子(VEGF)水平。于胎盘娩出后收集胎盘组织,检测胎盘circRNA表达情况,选取上调及下调基因各自差异表达水平由高到低排名前3位的基因进行分析;分析孕妇差异表达基因与24 h尿蛋白定量、平均动脉压、STOX1、sFlt-1、PLGF和VEGF的相关性;采用多元线性回归模型分析影响孕妇24 h尿蛋白定量的相关因素。结果与对照组比较,子痫前期组及重度子痫前期组的孕前体质量指数和平均动脉压升高(P<0.05);对照组、子痫前期组及重度子痫前期组24 h尿蛋白定量、STOX1、sFlt-1、胎盘hsa_circ_0014736、hsa_circ_0015383及hsa_circ_0004904表达水平依次升高,而hsa_circ_0063517、hsa_circ_0007121及hsa_circ_0003171表达水平依次降低(P<0.05);子痫前期组及重度子痫前期组hsa_circ_0014736、hsa_circ_0015383及hsa_circ_0004904分别与BMI、平均动脉压、24 h尿蛋白定量、STOX1、sFlt-1呈正相关,与VEGF、PLGF呈负相关(P<0.05),而hsa_circ_0063517、hsa_circ_0007121及hsa_circ_0003171与临床特征的相关性与此相反(P<0.05)。多元线性回归分析显示,hsa_circ_0015383与孕妇24 h尿蛋白定量呈正向线性相关,hsa_circ_0063517及hsa_circ_0007121与孕妇24 h尿蛋白定量呈负向线性相关(P<0.05)。结论子痫前期孕妇胎盘中hsa_circ_0063517及hsa_circ_0007121表达水平下调,hsa_circ_0015383表达水平上调,与患者病情密切相关。
Objective To analyze expression profile of circular RNA(circRNA)in placenta of patients with preeclampsia and its correlation with the patients'condition.Methods A total of 15 patients with preeclampsia(the preeclampsia group),25 patients with severe preeclampsia(the severe preeclampsia group),and 30 normal pregnant women(the control group)were selected.Clinical data of patients were collected.24 h urinary protein levels were detected in each group.Serum STOX1 protein,serum soluble vascular endothelial growth factor receptor 1(sFlt-1),placental growth factor(PLGF)and vascular endothelial growth factor(VEGF)were detected by enzyme-linked immunosorbent assay.The placental samples were collected after delivery,and the expression of circRNA in placenta was detected.The top three genes with differentially expressed levels of up-regulated and down-regulated genes from high to low were selected for analysis.The correlation of differentially expressed genes with 24 h urinary protein,mean arterial pressure,STOX1,sFlt-1,PLGF and VEGF in pregnant women was analyzed.Multiple linear regression model was used to analyze factors affecting the quantitative level of 24 h urine protein in pregnant women.Results Compared with the control group,there were higher prepregnancy body mass index and mean arterial pressure in the preeclampsia group and the severe preeclampsia group(P<0.05).The 24 h urinary protein quantity,STOX1,sFlt-1,hsa_circ_0014736,hsa_circ_0015383 and hsa_circ_0004904 expression levels in placenta were increased gradually in the control group,the preeclampsia group and the severe preeclampsia group.The expression levels of hsa_circ_0063517,hsa_circ_0007121 and hsa_circ_0003171 were decreased gradually(P<0.05).Values of hsa_circ_0014736,hsa_circ_0015383 and hsa_circ_0004904 were positively correlated with mean arterial pressure,24 h urinary protein quantity,STOX1 and sFlt-1 in the preeclampsia group and the severe preeclampsia group respectively.They were negatively correlated with VEGF and PLGF(P<0.05),while hsa_circ_0063517,hsa_circ_0007121 and hsa_circ_0003171 were negatively correlated with clinical features(P<0.05).Multiple linear regression analysis showed that hsa_circ_0015383 had positive linear correlation with 24 h urinary protein quantification in pregnant women,and hsa_circ_0063517 and hsa_circ_0007121 had negative linear correlation with 24 h urinary protein quantification in pregnant women(P<0.05).Conclusion The expression levels of hsa_circ_0063517 and hsa_circ_0007121 in placenta are down-regulated in preeclampsia pregnant women,and expression levels of hsa_circ_0015383 are up-regulated,which is closely related to the patient's condition.
作者
韩贞艳
关红琼
HAN Zhenyan;GUAN Hongqiong(Department of Obstetrics,Second Affiliated Hospital of Hainan Medical College,Haikou 570311,China)
出处
《天津医药》
CAS
北大核心
2023年第5期513-517,共5页
Tianjin Medical Journal
基金
海南省卫生健康行业科研项目(20A200470)。
关键词
先兆子痫
胎盘
RNA
环状
血管内皮生长因子受体-1
胎盘生长因子
血管内皮生长因子
24
h尿蛋白定量
pre-eclampsia
placenta
RNA,circular
vascular endothelial growth factor receptor-1
placenta growth factor
vascular endothelial growth factors
24 h urine protein quantification
