摘要
本试验以梨枣为材料,建立了高效液相色谱测定梨枣果皮酚类物质的色谱条件,色谱柱:大连依利特反相C18柱;紫外检测波长280 nm;柱温35℃;流速l mL/min;进样量为20μL;流动相A为甲醇,流动相B为超纯水(1%乙酸);梯度洗脱程序:0 min 5%A,40 min 30%A,45 min 100%A,55 min 100%A,60 min 5%A。酚类物质在40 min内得到了很好的分离,且回收率均较高,RSD值也较低。
This experiment with Lizao for the material of dates,established HPLC condition that seven phenolic compounds content synchronously,determinated:C18 chromatographic column;UV detection wavelength was 280 nm;Column temperature was 35℃;the flow rate was 1 mL/min;the injection volume was 20μL;Mobile phase A was methanol,mobile phase B is the ultra-pure water(1%acetic acid);Gradient elution program:0 min 5%A,40 min 30%A,45 min 100%A,55 min 100%A,60 min 5%A.Seven phenolic compounds in 40 min was well within the separation,and recovery rate were higher,the minimum detection limit is lower.
作者
王蕊
Wang Rui(Shanxi Yuncheng Vocational And Technical College Of Agricultural,Yuncheng,044000,China)
出处
《现代农业装备》
2023年第2期75-79,共5页
Modern Agricultural Equipment
关键词
梨枣
HPLC
酚类物质
方法
Lizao Jujube
HPLC
Phenolic compounds
methods
