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紫皮石斛花水提物对SD大鼠的生殖毒性研究

Reproductive toxicity study on water extract of flower of Dendrobium Devonianum Paxt.’s flower in SD rats
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摘要 目的对紫皮石斛花水提物进行生殖毒性评价。方法依照GB 15193.15—2015《食品安全国家标准生殖毒性试验》中一代生殖毒性评价方法,将240只9周龄SD大鼠(雌、雄各120只)按体质量随机分为0.0、2.5、5.0和15.0 g/kg∙BW组。实验中受试物经口灌胃给予。检测亲代(F0)大鼠在灌胃1~10周中的体质量、摄食、生殖脏器质量和系数;F0雄鼠精子参数以及F0雌鼠的动情周期、繁殖指数(受孕率和妊娠率)、胚胎毒性(子宫连胎质量、着床数、黄体数、活胎数、吸收胎数/率、死胎数/率);F1大鼠的哺乳期内存活率、哺乳期内体质量、肛殖距、每窝仔鼠平均数、仔鼠性别比;F1大鼠的青春期启动(睾丸下降、包皮分开、阴道开放)、体质量变化(3~12周龄)、生殖脏器质量和系数、精子参数;F0和F1大鼠所得脏器(包括子宫、卵巢、睾丸、附睾)进行组织病理学检查。结果3个剂量组的F0及F1大鼠的各生殖毒性指标与阴性对照组比较,均无显著性差异(P>0.05);组织病理学检查也没有发现显著变化。结论在本次实验条件下,紫皮石斛花水提物生殖毒性未观察到有害作用剂量(NOAEL)为15.0 g/kg∙BW。 Objective To evaluate the reproductive toxicity of water extract of flower of Dendrobium devonianum Paxt.Methods According to the reproductive toxicity evaluation from National Standard of the People’s Republic of China‘Reproductive Toxicity Study’(GB 15193.15—2015),240 nine-week-old SD rats(120 male and 120 female)were randomly grouped by body weight at a dose of 0.0,2.5,5.0 and 15.0 g/kg∙BW,respectively.SD rats were administrated by gavage.The detection included the body weight and and ingestion variations from 1st to10th week,reproductive organ weights and coefficients of F0 rats;the sperm parameters of F0 male rats,and estrous cycles,fertility index(conception and pregnancy rate),teratogenicity index(uterus weight,implantation number,corpus luteum number,living embryo number,resorption embryo number/rate and dead embryo number/rate)of F0 female rats;the survival rate and body weight variation during lactation period,anogenital distance,average litter size,sex ratio of F1 rats;the puberty onset(testis descending,foreskin separation,vaginal opening),body weight variation,reproductive organ weights and coefficients,and sperm parameters of F1 rats;the pathological examination on harvested organs of F0 and F1 rats including testis,epididymis,uterus and ovaries.Results Compared with control group,no significant difference in reproductive toxicity to F0 and F1 rats was observed in three dose groups(P>0.05).And no significant change was observed via histopathological examination.Conclusion In this reproductive test,the no observed adverse effect level(NOAEL)of water extract of the flower of Dendrobium devonianum Paxt.is 15.0 g/kg∙BW.
作者 邵乃敏 李能波 孙宇立 杨隽 徐菁 丁瑜 赵菊润 洪新宇 沈定才 SHAO Naimin;LI Nengbo;SUN Yuli;YANG Jun;XU Jing;DING Yu;ZHAO Jurun;HONG Xinyu;SHEN Dingcai(Shanghai Municipal Center for Disease Control&Prevention,Shanghai 200336,China;Longling Institute of Dendrobium,Yunnan Baoshan 678300,China)
出处 《中国食品卫生杂志》 CSCD 北大核心 2023年第2期190-197,共8页 Chinese Journal of Food Hygiene
关键词 紫皮石斛花水提物 SD大鼠 生殖毒性 Flower of Dendrobium devonianum Paxt. SD rats reproductive toxicity
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