摘要
目的根据美国临床和实验室标准协会(CLSI)药敏试验方法中的琼脂平板稀释法,对分离自西藏自治区鼠疫自然疫源地内的164株鼠疫耶尔森菌进行抗菌药物最低抑菌浓度的测定,监测西藏地区耐药鼠疫耶尔森菌株,掌握其抑菌范围,为鼠疫的临床治疗提供基础数据。方法利用琼脂平板稀释法分别测定氧氟沙星、环丙沙星、复方磺胺甲恶唑(甲氧苄啶-磺胺甲恶唑)、硫酸卡那霉素、链霉素、头孢曲松钠、氨苄青霉素钠、氯霉素、盐酸壮观霉素、头孢呋辛钠、盐酸四环素、莫西沙星共12种抗生素对164株鼠疫耶尔森菌的最低抑菌浓度(MIC)。若发现耐药菌株,则用K-B法进行验证。结果所测的164株鼠疫耶尔森菌中有1株对链霉素具有耐药性(MIC=4096μg/mL),链霉素K-B法检测抑菌环直径为0,这株菌对其余11种抗菌药物均敏感。其余163株菌对12种抗生素均敏感。结论发现1株鼠疫耶尔森菌对链霉素具有耐药性,这是我国首次发现鼠疫耶尔森菌对链霉素耐药。因链霉素是国内外治疗鼠疫的首选药物,耐药鼠疫耶尔森菌会引起链霉素治疗失败,从临床和公共卫生的角度来看,对鼠疫耶尔森菌分离株的抗菌敏感性监测应常规开展。
Objective According to the AGAR dilution method of drug sensitivity test method of the clinical and laboratory standards institute(CLSI),the minimal inhibitory concentration(MIC)of antimicrobial agents against Yersinia pestis isolated from plague natural foci in Tibet Autonomous Region was determined to monitor drug-resistant Yersinia pestis strains and master their inhibition range,so as to provide basic data for clinical treatment of plague.Methods The MIC of 12 antibiotics(ofloxacin,ciprofloxacin,trimethoprim-sulfamethoxazole,kanamycin sulfate,streptomycin,ceftriaxone sodium,ampicillin sodium,chloramphenicol,spectinomycin hydrochloride,cefuroxime sodium,tetracycline hydrochloride,and moxifloxacin)against 164 strains of Yersinia pestis were determined by the AGAR dilution method.If the resistant strain was found,the K-B method was used to verify the strain.Results One of 164 Yersinia pestis strains tested was resistant to streptomycin(MIC=4096μg/mL),and the diameter of inhibition ring detected by the streptomycin K-B method was 0.The strain was sensitive to the other 11 antibiotics.The other 163 strains were all sensitive to the 12 antibiotics.Conclusion One strain of Yersinia pestis was found to be resistant to streptomycin,which was the first time that Yersinia pestis was found to be resistant to streptomycin in China.Since streptomycin is the preferred drug for the treatment of plague at home and abroad,drug-resistant Yersinia pestis can cause streptomycin treatment failure.From the perspective of clinical and public health,the antimicrobial susceptibility monitoring of Yersinia pestis isolates should be carried out routinely.
作者
杨晓艳
辛有全
何建
靳娟
李胜
张琪
吴海莲
柏吉祥
金泳
代瑞霞
Yang Xiao-yan;Xin You-quan;He Jian;Jin Juan;Li Sheng;Zhang Qi;Wu Hai-lian;Bai Ji-xiang;Jin Yong;Dai Rui-xia(Professional Laboratory for Plague,Qinghai Institute for Endemic Disease Prevention and Control,Xi'ning 811602)
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2023年第3期357-360,I0003,共5页
Chinese Journal of Antibiotics
基金
青海省科技厅基础研究计划项目(No.2019-ZJ-7074)
国家卫生健康委鼠疫防治研究重点实验室(共建)(No.2019PT310004)
国家重点研发计划课题(No.2021YFC1200204)。
