摘要
目的分析赖氨酸甲基转移酶(SET domain containing 1A,SETD1A)、SET结构域5(SET domain-containing 5,SETD5)在乳腺癌中的表达及其与患者临床病理特征的相关性。方法收集2020年1月至2022年2月在东营市人民医院乳腺甲状腺外科就诊的80例乳腺癌患者,GSCA网站对SET结构域家族成员进行筛选,预测其在乳腺癌组织中的表达情况并借助免疫组化SP法进行验证。χ^(2)检验与Logistic回归模型分析SETD1A、SETD5与患者临床病理特征相关性。结果GSCA网站显示SET结构域家族的SETD1A、SETD5在乳腺癌组织中表达较正常组织上调(均P<0.05)。免疫组化SP法实验显示乳腺癌组织中SETD1A、SETD5阳性表达率分别为73.8%、68.8%,显著高于癌旁组织SETD1A、SETD5阳性表达率38.8%(χ^(2)=19.91,P<0.001)与32.5%(χ^(2)=21.03,P<0.001)。χ^(2)检验结果显示SETD1A的表达与与淋巴结转移、血管浸润显著相关,SETD5的表达与神经浸润显著相关(均P<0.05)。Logistic回归模型显示SETD1A表达与淋巴结转移(OR=0.07,95%CI:0.01~0.25,P<0.001)、分子分型(OR=0.04,95%CI:0.00~0.48,P=0.022)存在相关性,SETD5表达与神经浸润(OR=6.41,95%CI:1.45~46.65,P=0.029)存在相关性。结论组蛋白甲基转移酶SETD1A、SETD5在乳腺癌组织中表达上调,且与患者淋巴结转移、血管浸润、神经浸润等病理特征存在相关性。
Objective To analyze the expression of histone methyltransferase SETD1A and SETD5 in breast cancer and its correlation with the clinicopathological characteristics of patients.Methods A total of 80 breast cancer patients were included in the study.GSCA website screened SET domain family members,predicted their expression in breast cancer tissues,and verified them with immunohistochemical SP method.Chi-square test and Logistic regression model were used to analyze the correlation between SETD1A,SETD5 and clinicopathological characteristics of patients.Results The GSCA website showed that the expressions of SETD1A and SETD5 of the SET domain family were up-regulated in breast cancer tissues compared with normal tissues(all P<0.05).Immunohistochemical SP method showed that the positive expression rates of SETD1A and SETD5 in breast cancer tissues were 73.8%and 68.8%respectively,which were significantly higher than the positive expression rates of SETD1A and SETD5 in paracancerous tissues 38.8%(χ^(2)=19.91,P<0.001)and 32.5%(χ^(2)=21.03,P<0.001).Chi-square test results showed that the expression of SETD1A was significantly correlated with lymph node metastasis and vascular invasion,and the expression of SETD5 was significantly correlated with nerve invasion(all P<0.05).Logistic regression model showed that SETD1A expression was correlated with lymph node metastasis(OR=0.07,95%CI:0.01-0.25,P<0.001)and molecular type(OR=0.04,95%CI:0.00-0.48,P=0.022),SETD5 expression was correlated with neural invasion(OR=6.41,95%CI:1.45-46.65,P=0.029).Conclusion The expressions of histone methyltransferases SETD1A and SETD5 are up-regulated in breast cancer tissues,and they are correlated with pathological features such as lymph node metastasis,vascular invasion,and neural invasion.
作者
颜莘
延丽霞
毕研青
Yan Xin;Yan Lixia;Bi Yanqing(Department of Thyroid and Breast Surgery,Dongying People’s Hospital,Dongying 257091,China)
出处
《中华内分泌外科杂志》
CAS
2023年第2期185-189,共5页
Chinese Journal of Endocrine Surgery
基金
山东省中医药科技计划项目(2021Q040)。
