摘要
为调查江苏省不同地区牲畜棚蚊虫种群分布及虫媒病毒携带情况,2022年7—9月选择江苏省南京、宿迁、连云港、泰州和镇江5个地区的牲畜棚生境,用诱蚊灯法采集蚊虫样本,通过分子生物学方法检测蚊虫携带的病毒,测序后利用生物信息学软件对病毒序列进行分析。结果显示,采集到蚊虫4属5种共3795只,其中三带喙库蚊为优势蚊种,占采集蚊虫总数的85.71%。从南京、宿迁、连云港的三带喙库蚊样本中共检测出4株乙脑病毒,批阳性率为3.67%,其他病毒均未检出。对其中南京和宿迁2株乙脑病毒的E基因序列构建系统进化树,发现均属于基因Ⅰ-b型,与基因Ⅰ-b型毒株的核苷酸和氨基酸同源性分别在95.4%、97.0%以上。与疫苗株SA14-14-2在E蛋白上存在14个氨基酸差异位点,但是不在影响抗原抗体结合的区域。在毒力关键位点上与SA14-14-2疫苗株完全不同,与近几年江苏分离株却完全相同。结果表明,江苏省蚊虫仍具有乙脑传播风险,应加强蚊虫监测与防制工作。
Livestock sheds in Nanjing,Suqian,Lianyungang,Taizhou and Zhenjiang City,Jiangsu Province were selected to investigate the distribution of mosquito populations and arbovirus carried in livestock from July to September in 2022.Mosquito samples were collected by mosquito light trap method.Viruses carried by mosquitoes were screened by molecular biological methods and sequenced targeting on Flavivirus sp.and Alphavirus sp.The results showed that a total of 3795 mosquitoes of 5 species and 4 genera were collected in 5 areas of Jiangsu Province.Culex tritaeniorhynchus was the dominant species,accounting for 85.71%of the total mosquitoes collected.Four strains of Japanese encephalitis virus were detected from Cx.tritaeniorhynchus samples from Nanjing,Suqian and Lianyungang,with a positive groups rate of 3.67%.None of the other viruses were detected.No other Flavivirus or Alphavirus was achieved.The phylogenetic tree of E gene of two JEV strains from Nanjing and Suqian showed that they belonged to genotypeⅠ-b,and the homology of nucleotide and amino acid with genotypeⅠ-b was up to 95.4%and 97.0%,respectively.There were 14 amino acid sites on E protein different from vaccine strain SA14-14-2,but not in the domain that affected antigen-antibody binding.In the key sites of virulence,it was completely different from the SA14-14-2 vaccine strain,but identical with the Jiangsu strain in recent years.In conclusion,Cx.tritaeniorhynchus in Jiangsu Province still have the risk for JE transmission,and the mosquito surveillance and control should be strengthened.
作者
刘星宇
吴治明
高剑
陈红娜
杨维芳
褚宏亮
LIU Xing-yu;WU Zhi-ming;GAO Jian;CHEN Hong-na;YANG Wei-fang;CHU Hong-liang(School of Public Health,Nanjing Medical University,Nanjing 211166,Jiangsu,China;Department of Disinfection and Vector Control,Jiangsu Center for Disease Control and Prevention,Nanjing 210009,Jiangsu,China)
出处
《寄生虫与医学昆虫学报》
CAS
2023年第1期39-45,共7页
Acta Parasitologica et Medica Entomologica Sinica
基金
新发突发重大传染病病原微生物重点实验室资助。
关键词
蚊虫
监测
虫媒病毒
流行性乙型脑炎病毒
江苏省
Mosquito
Surveillance
Arbovirus
Japanese encephalitis virus
Jiangsu Province
