摘要
以黄果茄(Solanum xanthocarpum)果实为试材,采用超声波辅助乙醇提取法,对绿原酸的提取工艺进行研究。通过调整乙醇水溶液体积浓度、液体与物料的比值、超声时间、超声温度等4组单因素实验的数值及结果,优化出拟定的绿原酸提取工艺参数,即乙醇溶液浓度、液料比值、超声时间、超声温度分别为65%、50 mL/g、40 min、60℃。在上述单因素优化结果基础上,进一步通过Box-Behnken响应面法优化得到最终的二次多项数学模型。研究结果表明,提取工艺参数为64.7%的乙醇浓度、55.8 mL/g的液料比值、56.0℃下超声40.5 min,黄果茄绿原酸提取率最佳,为6.81%。黄果茄果中的提取物具有较好的抗氧化能力,相应的1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-pierylhydrazy,DPPH)和2,2-连氮基-双-(3-乙基苯并二氢噻唑啉-6-磺酸)[2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate),ABTS]自由基清除能力的IC50分别为55.7 mg/L和20.1 mg/L。
Solanum xanthocarpum fruits are used as the test material,and the extraction process of chlorogenic acid is studied in detail by using the ultrasonic-assisted ethanol extraction test method.Values of four group parameters for the volume solubility of ethanol aqueous solution,ratio of liquid to the material,ultrasonic time,ultrasonic temperature were adjusted,and then the proposed extraction process parameters of chlorogenic acid from single-factor experiments and the corresponding results were optimized.The optimized parameters were ethanol concentration 65%,ratio of liquid to material 50 mL/g,ultrasonic temperature 60℃and ultrasonic time 40 min.On the basis of the above single factor optimization results,the final quadratic multiphase mathematical model was obtained further by optimization from the Box-Behnken response surface method.The extraction process parameters were ethanol concentration 64.7%,liq-uid-to-solid ratio 55.8 mL/g,and ultrasonic temperature 56.0℃for 40.5 min,and the optimal extraction rate of chlorogenic acid was 6.81%.The extract of S.xanthocarpum fruits had good antioxidant capacity to DPPH and ABTS free radical scavenging with IC50 values of 55.7 mg/L and 20.1 mg/L,respectively.
作者
郭蒙
郭纯
蒋青
高林晓
GUO Meng;GUO Chun;JIANG Qing;GAO Linxiao(School of Chemistry and Chemical Engineering,Qiannan Normal University for Nationalities,Duyun,Guizhou 558000,China;Guizhou Province College Research Center of Ethnical Medicinal Plant Resources Exploitation Engineering,Duyun,Guizhou 558000,China)
出处
《热带作物学报》
CSCD
北大核心
2023年第4期825-833,共9页
Chinese Journal of Tropical Crops
基金
贵州省教育厅项目(黔教合KY字[2018]440)
国家级大学生创新创业训练项目(No.202110670005)
贵州省普通高校民族药用植物资源开发工程研究中心开放基金(黔教合KY字[2014]227)。
关键词
黄果茄
绿原酸
超声波辅助提取工艺
工艺优化
抗氧化活性
Solanum xanthocarpum
chlorogenic acid
ultrasound-assisted extraction process
process optimization
antioxidant activity
