摘要
骨骼肌生长发育对肉鸡产业至关重要,可变剪切(AS)作为生物体内一种普遍存在的调控机制,在骨骼肌发育过程中发挥着重要作用。本研究以快长型、慢长型边鸡品系为试验材料,收集受精蛋后孵化至14胚龄和20胚龄,然后采集鸡胚腿肌进行转录组测序,用于分析骨骼肌发育过程中的差异可变剪切事件。在14胚龄慢长型(S14)与14胚龄快长型(F14)比较组中共发生230个差异可变剪切事件,其对应了200个基因,在20胚龄慢长型(S20)与20胚龄快长型(F20)比较组中共发生373个差异可变剪切事件,其对应了324个基因,且2个比较组中存在47个相同的基因。GO富集分析结果表明,前20个富集条目中包括丝氨酸家族氨基酸分解代谢过程、肌球蛋白II细丝组装和肌动球蛋白结构组织等与骨骼肌发育相关的生物学过程。KEGG通路富集分析结果表明,前20条通路中有多条通路与骨骼肌发育相关,包括黏附连接、肌动蛋白细胞骨架调节和黏着斑等。基于STRING数据库对所有差异可变剪切事件的来源基因进行蛋白质互作网络分析,然后利用Cytoscape(3.8.2)软件中的插件CytoHubba筛选核心基因,结合富集分析结果在S14与F14比较组中发现TLN2、PARVB和ITGA6等多个关键候选基因,在S20与F20比较组中发现LDB3、PDLIM3、ITGB5、DMD、TNS3和RAC1等多个关键候选基因,同时,筛选到的PDLIM5和GIT1候选基因在14胚龄、20胚龄边鸡骨骼肌发育中均发挥重要作用。本研究结果为进一步解析骨骼肌发育调控机理奠定了基础,也为黄羽肉鸡的选育工作提供一定参考。
The growth and development of skeletal muscle is vital to the broiler industry.Alternative splicing(AS)is a universal regulation mechanism in organisms and plays an important role in the development of skeletal muscle.The fast-growing and slow-growing groups of Bian chicken were used as trial materials.After the fertilized eggs were collected and incubated to 14 and 20 days,the leg muscles of Bian chickens were collected for RNA-seq.Results showed that 230 and 373 differentially expressed alternative splicing events(DEAS)were found in S14(14-day embryos of slow-growing chicken)vs F14(14-day embryos of fast-growing chicken)and S20(20-day embryos of slow-growing chicken)vs F20(20-day embryos of fast-growing chicken)groups,corresponding to 200 and 324 host genes respectively.There were 47 same host genes in the two comparison groups.GO analysis results showed that biological processes(BP)terms related to skeletal muscle development in the top 20,such as serine family amino acid catabolic process,myosin II filament assembly and actomyosin structure organization,were obtained.KEGG enrichment analysis found that some of the top 20 pathways were also related to skeletal muscle development,including adherens junction,regulation of actin cytoskeleton and focal adhesion.Protein-protein interaction network(PPI)analysis was performed for the host genes of all the DEAS.Key candidate host genes were further screened using the plug-in CytoHubba combined with functional enrichment results.Key candidate host genes TLN2,PARVB and ITGA6 were found in S14 vs F14 group,and LDB3,PDLIM3,ITGB5,DMD,TNS3 and RAC1 were found in S20 vs F20 group.The results also showed that PDLIM5 and GIT1 were both important in the two comparison groups.These results can lay a foundation for further understanding and analyzing the regulation mechanism of skeletal muscle development,and also provide a reference for the breeding of yellow-feathered broilers.
作者
魏清宇
吴鹏飞
叶红心
李培峰
崔少华
张旗
张丽
张跟喜
WEI Qing-yu;WU Peng-fei;YE Hong-xin;LI Pei-feng;CUI Shao-hua;ZHANG Qi;ZHANG Li;ZHANG Gen-xi(College of Animal Science,Shanxi Agricultural University,Taiyuan 030032,China;College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China)
出处
《江苏农业学报》
CSCD
北大核心
2023年第2期444-452,共9页
Jiangsu Journal of Agricultural Sciences
基金
山西省重点研发项目(201703D221022-3)
生物育种工程项目(yzgc129)
国家肉鸡产业技术体系项目(CARS-41)
江苏高校优势学科建设工程项目(PAPD)
山西省现代农业产业技术体系建设专项资金资助项目(2022-07)。
关键词
骨骼肌
可变剪切
鸡
skeletal muscle
alternative splicing
chicken
