摘要
采用反相微乳液法制备了含有上转换纳米粒子Na YF_(4)∶Yb,Tm@NaGdF4∶Yb、聚多巴胺(PDA)、聚乙烯亚胺(PEI)和MnO_(2)的多功能纳米光敏剂(UPM),借助TEM、FTIR、XPS、UV-Vis、PL和DLS表征了产物的形貌、化学组成、光学性质和粒径分布,测试了UPM的活性氧物种产生能力、对盐酸四环素(TC)的负载与释放性能,考察了负载TC的光敏剂(UPM/TC)在近红外(NIR)光照下的抗菌性能。结果表明,UPM具有类过氧化氢酶活性,在H_(2)O_(2)存在和NIR光照下可产生羟基自由基与单线态氧。UPM/TC的载药量为22.6%,体外释放具有pH响应特征,在pH为5.0的磷酸盐缓冲液中24 h累积释药率为87.1%。在功率密度1.2 W/cm^(2)的980 nm NIR光照射10 min后,质量浓度为0.8 g/L的UPM/TC对大肠杆菌的杀菌率达到99.7%,抗菌效果优于TC或UPM,呈现出光动力-抗生素协同抗菌效应。
Multifunctional nanophotosensitizers(UPM)composing of upconversion nanoparticles NaYF_(4)∶Yb,Tm@NaGdF4∶Yb,polydopamine,polyethyleneimine and MnO_(2) were prepared by reverse microemulsion method.The morphology,chemical composition,optical properties and size distribution of the products obtained were then characterized by TEM,FTIR,UV-Vis,PL and DLS.Next,the reactive oxygen species generation ability as well as the loading and release of tetracycline hydrochloride(TC)from UPM were measured,and the antibacterial performance of TC-loaded nanophotosensitizers(UPM/TC)under nearinfrared(NIR)light irradiation was investigated.The results showed that UPM exhibited catalase-like activity and could produce hydroxyl radical and singlet oxygen in presence of H_(2)O_(2) when exposed to NIR light.The loading capacity of TC in UPM was 22.6%,and the in vitro release of TC was pH-sensitive.The cumulative release rate of UPM was 87.1%in phosphate buffer at pH 5.0 for 24 h.When exposed to 980 nm NIR light(1.2 W/cm^(2))for 10 min,UPM/TC at a mass concentration of 0.8 g/L exhibited 99.7%bacterial inactivation for E.coli and better antibacterial performance than free TC or UPM by virtue of the synergistic effect of photodynamic and antibiotic sterilization.
作者
靳丹丹
曹潇楠
俞彬
赵品章
萧睿骞
宫培军
JIN Dandan;CAO Xiaonan;YU Bin;ZHAO Pinzhang;XIAO Ruiqian;GONG Peijun(Key Laboratory of the Ministry of Education for Advanced Catalysis Materials,College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua 321000,Zhejiang,China)
出处
《精细化工》
EI
CAS
CSCD
北大核心
2023年第4期802-811,共10页
Fine Chemicals
基金
浙江省公益技术应用研究计划项目(LGF20E020001)
浙江省新苗人才计划项目(2022R404B054)。
关键词
上转换发光
二氧化锰
药物负载
光动力治疗
类过氧化氢酶活性
抗菌性能
功能材料
upconversion luminescence
manganese dioxide
drug loading
photodynamic therapy
catalaselike activity
antibacterial properties
functional materials
