摘要
为探究科尔沁沙地紫花苜蓿抗寒性对壳聚糖施用水平的响应,以‘公农1号’紫花苜蓿为材料,采用单因素随机区组试验设计,于秋季喷施含量为0、50、100、150、200μg·g^(-1)的壳聚糖,将紫花苜蓿越冬材料模拟低温冷冻条件(-20℃)和冷藏(4℃)处理,检测紫花苜蓿根颈的相对电导率以及可溶性蛋白、脯氨酸、蔗糖和果糖含量的变化。结果表明,在低温冷冻条件下,100μg·g^(-1)壳聚糖处理时,紫花苜蓿根颈的相对电导率最低,为31.49%,而脯氨酸、蔗糖和果糖含量均为最高,分别为8.05、88.17和86.32μg·g^(-1);150μg·g^(-1)壳聚糖处理时,紫花苜蓿根颈的可溶性蛋白含量最高,为27.19 mg·g^(-1)。相关性分析结果表明,不同低温胁迫处理下紫花苜蓿根颈中可溶性蛋白、脯氨酸、蔗糖、果糖与相对电导率的关系均为负相关。在低温冷冻胁迫条件下,壳聚糖通过提高苜蓿根颈的可溶性蛋白、脯氨酸、蔗糖、果糖含量,可以降低相对电导率,提高苜蓿抗寒性。在科尔沁沙地秋季喷施100~150μg·g^(-1)壳聚糖有利于苜蓿安全越冬。
In order to explore the response of alfalfa cold resistance to chitosan application level in Horqin sandy land,taking‘Gongnong 1’alfalfa variety as the material,a single factor randomized block experiment design was adopted,and 0,50,100,150,200μg·g^(-1)amount of chitosan were sprayed in autumn.The alfalfa overwintering materials were treated under simulated low-temperature freezing conditions(–20℃)and cold storage(4℃),and the changes of relative conductivity of alfalfa root collar and the contents of soluble protein,proline,sucrose and fructose were detected.The results showed that under the condition of low temperature freezing,when treated with 100μg·g^(-1)chitosan,the relative conductivity of alfalfa root collar was the lowest,which was 31.49%,while the contents of proline,sucrose and fructose were the highest,which were 8.05,88.17 and 86.32 mg·g^(-1),respectively.When treated with 150μg·g^(-1)chitosan,the soluble protein content of alfalfa root collar was the highest,which was 27.19 mg·g^(-1).The results of correlation analysis showed that the relationships between soluble protein,proline,sucrose,fructose and relative conductivity were negative under different low temperature stress treatments.Under the condition of low temperature freezing stress,chitosan could reduce the relative conductivity and improve the cold resistance of alfalfa by increasing the contents of soluble protein,proline,sucrose and fructose in the root collar of alfalfa.Spraying 100~150μg·g^(-1)chitosan in Horqin sandy land in autumn was conducive to the safe overwintering of alfalfa.
作者
滕泽
张玉霞
陈卫东
丛百明
田永雷
张庆昕
张永亮
王东儒
TENG Ze;ZHANG Yuxia;CHEN Weidong;CONG Baiming;TIAN Yonglei;ZHANG Qingxin;ZHANG Yongliang;WANG Dongru(College of Agriculture,Inner Mongolia University for Nationalities,Inner Mongolia Tongliao 028041,China;Institute of Ecological Environment and Green Development,Inner Mongolia University for Nationalities,Inner Mongolia Tongliao 028000,China;Institute of Animal Husbandry and Veterinary Science of Tongliao City,Inner Mongolia Autonomous Region,Inner Mongolia Tongliao 028000,China;Inner Mongolia Autonomous Region Academy of Industrial Sciences,Hohhot 010010,China;Agricultural and Animal Husbandry Technology Extension Center of Inner Mongolia Autonomous Region,Hohhot 010010,China)
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2023年第2期192-198,共7页
Journal of Agricultural Science and Technology
基金
内蒙古自治区自然科学研究基金项目(2020MS03081)
国家自然科学基金项目(31960352)
内蒙古自治区科技计划项目(2021GG0109)
国家牧草产业技术体系(CARS-34)。
关键词
壳聚糖
紫花苜蓿
低温胁迫
抗寒性
chitosan
alfalfa
low temperature stress
cold resistance
