摘要
GbRLCK10为海岛棉响应黄萎病菌和激素胁迫基因。为研究该基因在烟草中的功能,利用农杆菌介导法将其导入烟草。对获得的阳性植株通过室内接种法进行抗病性鉴定,并利用qRT-PCR技术分析水杨酸途径关键基因NtPR1、NtNPR1和乙烯途径关键基因NtEIN4、NtNTHK2及茉莉酸途径关键基因NtJAZ1、NtAOS的表达特征。结果表明,接种前,转基因植株中NtPR1、NtNPR1、NtJAZ1基因的表达量显著高于野生型植株;而野生型植株中NtEIN4、NtNTHK2、NtAOS基因表达量显著高于转基因植株。接种黄萎病菌后,转基因植株的病情指数显著高于野生型;野生型植株感病后,NtPR1、NtNPR1、NtAOS基因的表达量显著高于发病前,而NtJAZ1、NtEIN4、NtNTHK2基因的表达量显著低于发病前;转基因植株感病后,NtPR1、NtNPR1、NtEIN4、NtAOS基因的表达量均显著高于发病前,而NtJAZ1、NtNTHK2基因的表达量均显著低于发病前;与野生型相比,转基因植株NtPR1、NtAOS基因的表达量显著降低,而NtNPR1、NtEIN4、NtJAZ1、NtNTHK2基因的表达量显著提高。综上所述,GbRLCK10基因通过调控激素途径中关键基因的表达水平影响烟草黄萎病抗性,为进一步揭示GbRLCK10基因对棉花黄萎病响应机制的功能研究提供了重要依据。
GbRLCK10 gene responsed to Verticillium wilt and hormone stress in Gossypium barbadense.To analysis the function of GbRLCK10 gene,it was introduced into tobacco by Agrobacterium mediated method,and the positive transgenic plants were got.The disease resistances of the transgenic plants were identified in greenhouse,and the expressions of salicylic acid pathway key genes(NtPR1 and NtNPR1),ethylene pathway genes(NtEIN4 and NtNTHK2),and jasmonic acid pathway genes(NtJAZ1 and NtAOS)were analyzed by qRT-PCR.The results showed that,before infection,the expressions of NtPR1,NtNPR1 and NtJAZ1 genes in transgenic plants were significantly higher than those in wild type,and the expressions of NtEIN4,NtNTHK2 and NtAOS genes in wild type were significantly higher than those in transgenic plants.After inoculation with Verticillium wilt,the disease index of transgenic plants was significantly higher than that of wild type.And after the wild-type plants were infected,the expressions of NtPR1,NtNPR1 and NtAOS genes were significantly higher than those of the control,while the expressions of NtEIN4,NtNTHK2 and NtJAZ1 genes were significantly lower than those of the control;after transgenic plants were infected,the expression levels of NtPR1,NtNPR1,NtEIN4 and NtAOS genes were significantly higher than those in the control,while the expression levels of NtJAZ1 and NtNTHK2 genes were significantly lower than those in the control.Compared with the wild type,the expressions of NtPR1 and NtAOS genes in transgenic plants were significantly lower,while the expressions of NtNPR1,NtEIN4,NtJAZ1 and NtNTHK2 genes were significantly higher than those in the wild type.In conclusion,GbRLCK10 gene affected tobacco Verticillium wilt resistance by regulating the expressions of key genes in hormone pathway,which provided important basis for further revealing the function of GbRLCK10 gene in response to cotton Verticillium wilt.
作者
赵曾强
张国丽
马盼盼
李有忠
王志军
谢宗铭
孙国清
ZHAO Zengqiang;ZHANG Guoli;MA Panpan;LI Youzhong;WANG Zhijun;XIE Zongming;SUN Guoqing(Xinjiang Production&Construction Group Key Laboratory of Crop Germplasm Enhancement and Gene Resources Utilization,Biotechnology Research Institute,Xinjiang Academy of Agricultural Reclamation Sciences,Xinjiang Shihezi 832000,China;Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2023年第3期57-65,共9页
Journal of Agricultural Science and Technology
基金
兵团重点领域创新团队建设计划项目(2019CB008)
八师石河子市中青年科技创新领军人才计划项目(2020RC06)。
