摘要
为研究接种根瘤菌对甘草主要活性成分的影响,提高甘草质量与产量及开发优质菌剂,以无处理甘草为对照,将3种根瘤菌(Rhizobium mongolense subsp.loessense strain CCBAU 7190B、Agrobacterium tumefaciens IAM13129、Agrobacterium tumefaciens strain CCBAU 65237)菌液按照10 mL·株^(-1)(675×10^(-6)CFU·mL^(-1))接种于乌拉尔甘草、光果甘草、胀果甘草的地下部分,采用高效液相色谱法(high performance liquid chromatography,HPLC)测定不同处理下甘草的甘草酸、甘草苷、甘草素、异甘草苷及异甘草素含量。结果表明,在3种菌株处理后,乌拉尔甘草、光果甘草和胀果甘草中5种活性成分含量均显著高于对照组(P<0.05)。由此表明,根瘤菌与甘草共生可以显著提高甘草酸、甘草苷、甘草素、异甘草苷、异甘草素含量的积累,为提高甘草质量与产量提供参考。
In order to study the effects of rhizobia on main active ingredients of three kinds of licorice,improve the quality and yield of licorice and develop a high quality rhizobia bacteria,three kinds of rhizobias(Rhizobium mongolense subsp.loessense strain CCBAU 7190B,Agrobacterium tumefaciens IAM 13129,Agrobacterium tumefaciens strain CCBAU 65237)were inoculated in the underground part of three types of licorices(Glycyrrhiza uralensis Fisch.,G.glabra L.and G.inflata Bat.)with 10 mL·plant^(−1)(675×10^(−6) CFU·mL^(−1)),and the untreated hay as the control.The contents of glycyrrhizic acid,liquiritin,liquiritigenin,isoliquiritin and isoliquiritigenin were determined by high performance liquid chromatography(HPLC).The results showed that,compared with control,rhizobia treatment increased significantly the contents of 5 active ingredients in Glycyrrhiza uralensis Fisch.,G.glabra L.and G.inflata Bat..This suggested that rhizobia-licorice symbiosis could significantly increase the accumulation of glycyrrhizic acid,liquiritin,liquiritigenin,isoliquiritin and isoliquiritigenin,which provided reference for improving the quality and yield of licorice.
作者
丁晓霞
马生军
陈文峰
张澳港
于宏超
依里汗木江·加帕尔
DING Xiaoxia;MA Shengjun;CHEN Wenfeng;ZHANG Aogang;YU Hongchao;Yilihanmujiang Jiapaer(College of Food and Pharmaceutical Science,Xinjiang Agricultural University,Urumqi 830052,China;Research Center of Biological Sciences and Rhizobia,College of Biological Sciences,China Agricultural University,Beijing 100193,China)
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2023年第3期212-220,共9页
Journal of Agricultural Science and Technology
基金
新疆维吾尔自治区研究生科研创新项目(XJ2021G173)
中农新农联合基金项目(2519-ZNXN19-H)。
关键词
根瘤菌
甘草
活性成分
多元统计分析
rhizobia
licorice
active ingredient
multivariate statistical analysis
