摘要
蛋白基生物材料具有生物相容性高、降解能力强、免疫反应低等特点,是近几年生物材料领域研究的又一热点。利用基因编辑结合合成生物学技术手段,可通过蛋白质重组,构建人工设计的重组蛋白生物材料,不仅可弥补天然蛋白在生物活性、理化性质等方面的不足,还可以人工修饰引入特定功能基团,极大扩展蛋白基生物材料的发展和应用。丝蛋白、弹性蛋白和胶原蛋白作为蛋白基生物材料常用的骨架蛋白,可通过基因编辑技术实现其对功能、理化性质等特性的精确控制。本文分别对丝蛋白、弹性蛋白、胶原蛋白的基本构建原理、生物合成与纯化方法等进行综述,探讨其在组织工程、药物递送领域中的应用,展望其发展,为开展相关研究提供基本参考。
Due to the advantages in biocompatibility,biodegradables and immune responses,protein-based biomaterials have attracted attations of researchers from both biomedical and biomaterials fields.The combination of genetic editing and biosynthesis technologies allow people to construct recombination proteins and utilize them in biomaterials fabrications.Importantly,the recombinant proteins can compensate for deficiencies of the biomaterials fabricated by natural proteins,such as the poverty of bio-functions and disadvantages of mechanical properties of natural proteins.Moreover,the recombinant proteins can be precisely controlled to introduce bio-activity groups and improve their mechanical properties.Thus,the development of genetically encoded protein-based biomaterials extend the potential applications of protein biomaterials.Herein,this review summarizes recently developed recombinant silk-like proteins,elastin-like proteins,and collagen-like proteins.The constructions,biosynthesis,and purification methods of those recombinant proteins were demostracted.Advanced applications in biomedical research have also been reviewed.This review will provide a basic reference for the development of genetically encoded protein-based biomaterials.
作者
李依苗
王洁琦
王小杰
王杰
贾爽
李昕宇
LI Yimiao;WANG Jieqi;WANG Xiaojie;WANG Jie;JIA Shuang;LI Xinyu(School of Life Science,Inner Mongolia University,Hohhot 010070,China;Inner Mongolia Hecent Biotech Company,Hohhot 010070,China)
出处
《内蒙古大学学报（自然科学版）》
CAS
北大核心
2023年第2期213-224,共12页
Journal of Inner Mongolia University：Natural Science Edition
关键词
基因编辑
生物材料
组织工程
药物递送
重组蛋白
gene editing
biomaterials
tissue engineering
drug delivery
recombination protein
