摘要
目的基于实时体温监测技术比较酵母菌、脂多糖(LPS)、2,4-二硝基酚对小鼠致热作用及呼吸功能的影响。方法SPF级C57BL/6J雌性小鼠12只,进行动物核心体温监测胶囊腹腔埋入,将小鼠按体重、体温随机分为空白对照组、酵母菌组、LPS组、2,4-二硝基酚组。小鼠分别注射相应发热介质后,每天记录动物的体重,动物核心体温监测胶囊的记录频率为:每次15 min。3 d后对小鼠进行无创呼吸监测,分别测定特殊气道阻力(sRaw)、潮气量(TV)、呼吸频率(F)和气道传导率(sGaw)。实验结束后对小鼠进行解剖,取其胸腺、脾、肺进行称重并计算脏器指数。结果实验过程中,空白对照组小鼠体重持续上升,酵母菌组、2,4-二硝基酚组小鼠体重持续下降,LPS组小鼠48 h内体重升高,48 h后体重下降。酵母菌组脾指数对比空白对照组极显著升高(P<0.01),酵母菌组、2,4-二硝基酚组的胸腺指数对比空白对照组显著降低(P<0.05)。酵母菌组小鼠体温在注射后3 h开始升高,在48 h内持续处于高温水平,在24~48 h期间体温达到最大值,与空白对照组比,差异具有极显著性(P<0.01);48~72 h期间,酵母菌组小鼠体温开始逐渐下降,与空白对照组之间差异无显著性(P>0.05)。LPS组小鼠体温在注射后4 h开始升高,高温状态维持至8 h后开始降低,但仍高于空白对照组。无创呼吸监测发现,酵母菌组、LPS组、2,4-二硝基酚组sRaw均出现升高,TV、F、sGaw均出现降低。结论从发热时长比较,酵母菌作为发热介质引起发热时间最久,其次为LPS、2,4-二硝基酚;以发热趋势比较,酵母菌表现出先降后升的状态,于注射10 h达到峰值状态,LPS表现为持续升温,于注射4 h达到峰值状态,2,4-二硝基酚表现为先降后升,于注射8 h达到峰值状态;从呼吸功能比较,小鼠注射发热介质后,均出现气道阻力增强、抑制呼吸频率、降低潮气量、气道传导减弱情况,其中对比3种发热介质对小鼠呼吸影响,酵母菌对小鼠呼吸频率、潮气量、气道阻力、气道传导作用较强。因此,酵母菌无论是发热时长、发热高峰以及对机体呼吸功能抑制作用都更显著。
Objective To compare the effects of yeast,lipopolysaccharide(LPS),and 2,4-dinitrophenol on fever and respiratory functions in mice by a real-time temperature monitoring technique.Methods Twelve SPF C57BL/6J female mice were implanted intraperitoneally with animal core body temperature monitoring capsules.The mice were randomly divided into blank control,yeast,LPS,and 2,4-dinitrophenol groups by their body weight and temperature.After injection of the corresponding heating medium,body weight was recorded every day,and the recording frequency of the animal core body temperature monitoring capsule was times 15 min.Three days later,non-invasive respiratory monitoring was performed in mice,and specific airway resistance(sRaw),tidal volume(TV),respiratory rate(F),and airway conductivity(sGaw)were measured.At the end of the experiment,the mice were dissected,their thymus,spleen,and lungs were weighed,and organ indexes were calculated.Results During the experiment,body weight of the blank control group continued to increase,body weight in yeast and 2,4-dinitrophenol groups continued to decrease,and body weight in the LPS group increased within 48 h and decreased after 48 h.There was a hightly significant difference in the spleen index between normal and yeast groups(P<0.01),and there were significant differences in thymus index between normal and yeast groups,and between normal and 2,4-dinitrophenol groups(P<0.05).Body temperature in the yeast group began to increase at 3 h after injection,remained high within 48 h,and reached the maximum during 24~48 h,which was statistically different from that in the blank control group(P<0.01).From 48 to 72 h,body temperature in the yeast group began to decrease gradually,and there was no statistical difference between yeast and blank control groups(P>0.05).Body temperature in the LPS group increased at 4 h after injection and decreased at 8 h after injection,but it remained higher than that in the blank control group.Non-invasive respiratory monitoring showed that sRaw in yeast,LPS,and 2,4-dinitrophenol groups was increased,while TV,F,and sGaw were decreased.Conclusions From comparisons of the febrile duration,yeast as the heating medium caused the longest febrile duration,followed by LPS and 2,4-dinitrophenol.By comparing fever trends,yeast induced a state of first decreasing and then increasing temperature,reaching the peak at 10 h after injection,LPS induce a state of continuous warming,reaching the peak at 4 h after injection,and 2,4-dinitrophenol induced a state of first decreasing and then increasing temperature,reaching the peak at 8 h.From the comparison of respiratory functions,mice injected with the heating medium showed increased airway resistance,an inhibited respiratory rate,reduced tidal volume,and weakened airway conduction.By comparing the effects of the three heating media on respiration,yeast had strongest effects on the respiratory rate,tidal volume,airway resistance,and airway conduction.Therefore,yeast has a more significant effect on the duration and peak of fever and inhibits respiratory functions.
作者
晋一帆
李胜群
孙竹筠
刘孝云
叶冬雪
赵涛
肖敏
容蓉
杨勇
JIN Yifan;LI Shengqun;SUN Zhuyun;LIU Xiaoyun;YE Dongxue;ZHAO Tao;XIAO Min;RONG Rong;YANG Yong(Shandong University of Traditional Chinese Medicine,Jinan 250355;China.2.Lunan Pharmaceutical Group Co,Linyi 276006;.3.Linyi Hospital of Traditional Chinese Medicine,Linyi 276000)
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2023年第4期454-462,共9页
Acta Laboratorium Animalis Scientia Sinica
基金
山东省重点研发计划(重大科技创新工程)(2020CXGC010505)
国家自然科学基金(82274397)
山东省自然科学基金(ZR2021LZY012)
济南市“新高校20条”资助项目(自主培养创新团队)(2021GXRC028)。
关键词
发热模型
C57BL/6J小鼠
酵母菌
脂多糖
2
4-二硝基酚
植入子体温检测胶囊
呼吸功能
fever model
C57BL/6J mice
yeast
lipopolysaccharide
2,4-dinitrophenol
implant body temperature detection capsule
function of respiration Conflicts of Interest:The authors declare no conflict of interest
