摘要
为了解H11N3亚型禽流感病毒(AIV)的生物学特性,本研究对2020年在福州市某活禽市场鸭中分离的一株H11N3亚型AIV进行了全基因组测序、遗传进化分析和对BALB/c小鼠的致病性试验。测序结果显示,分离株HA裂解位点为PAIASR↓GLF,符合低致病性AIV分子特征;HA蛋白226L和228S突变显示病毒具有结合人源受体(SAα-2,6 Gal)的特征性氨基酸。PB1、PA、NP和M1蛋白均出现对哺乳动物致病性增强的特征性氨基酸。遗传进化分析显示,H11N3 AIV的8个基因节段均属于欧亚分支,其HA基因与A/duck/Fujian/SD061/2017(H11N3)株HA基因序列相似性最高,而NA基因与A/EN/Fujian/02754/2016(H3N3)株NA基因序列相似性最高。该病毒内部基因分别与浙江、福建和云南等地鸭和鸡体内分离的H7N9、H7N7、H1N2、H7N2、H7N7和H9N6等亚型AIV相关基因高度同源。小鼠致病性试验结果显示,该病毒未经适应即可在小鼠鼻甲和肺脏中高效复制,表明该病毒株具备感染哺乳动物的能力。上述结果表明该病毒株为新型重组病毒,对小鼠具有一定致病性,本研究为了解H11N3亚型AIV的生物学特性及其感染的防控提供了参考。
To investigate the biological characteristics of the H11N3 avian influenza virus(AIV),an H11N3 subtype AIV isolated from a duck in Fuzhou live poultry market in 2020 was used for full genome sequencing,genetic evolution analysis,and pathogenicity test in BALB/c mice.The sequence analysis of the hemagglutinin(HA)indicated that the virus was categorized to low pathogenic AIV,with the cleavage site of PAIASR↓GLF.The virus may bind toα-2,6-linked sialic acids(human-type receptor)with the distinctive amino acid of 226L and 228S of HA protein.In addition,PB1,PA,NP,and M1 proteins have amino acids that enhanced pathogenicity to mammals at particular positions.The phylogenic analysis showed that the eight gene segments clustered into Eurasian avian lineage.The HA and NA gene have high homology with that of A/duck/Fujian/SD061/2017(H11N3)and A/EN/Fujian/02754/2016(H3N3),respectively.The internal genes of the H11N3 virus share highly homologies with relative genes of H7N9,H7N7,H1N2,H7N2,H7N7,and H9N6 AIVs isolated from ducks and chickens in Zhejiang,Fujian,and Yunnan.The results of mouse pathogenicity test showed that the virus could replicate efficiently in the nasal turbinates and lungs of mice without adaptation,indicating that the H11N3 virus had the capacity to infect mice.These results indicated that the isolate was a new reassortant and showed low pathogenic to mice.In conclusion,this study laid the foundation for research on biological characteristics of H11N3 AIV and provided scientific basis for prevention and control of AIV.
作者
王辰川
李菁
文法鑫
陈福再
王露露
张春萍
黄佳承
杨洲
陈吉龙
马树杰
WANG Chen-chuan;LI Jing;WEN Fa-xin;CHEN Fu-zai;WANG Lu-lu;ZHANG Chun-ping;HUANG Jia-cheng;YANG Zhou;CHEN Ji-long;MA Shu-jie(Key Laboratory of Animal Pathogen Infection and Immunology of Fujian Province,College of Animal Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fujian-Nepal Joint Laboratory of Animal Pathogen Prevention and Control,College of Animal Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Key Laboratory of Fujian-Taiwan Animal Pathogen Biology,College of Animal Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,China;CAS Key Laboratory of Pathogenic Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences(CAS),Beijing 100101,China)
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2023年第2期115-121,共7页
Chinese Journal of Preventive Veterinary Medicine
基金
福建省中青年教师教育科研项目(JAT190126)
福建农林大学杰出青年科研人才计划项目(xjq202018)
国家重点研发计划(2021YFD1800205)。
