摘要
目的探讨脓毒症患者外周血白细胞介素(IL)-6、肿瘤坏死因子(TNF)-α和降钙素原(PCT)水平与IL-6基因启动子区-572C/G多态性的关系。方法回顾性纳入2018年10月~2020年10月湖北医药学院附属太和医院收治的脓毒血症患者135例为试验组,以住院期间是否发生休克将试验组患者分为脓毒症组(63例)和脓毒症休克组(72例),选取同期于我院体检健康人群148例为对照组。根据基因型再将试验组患者分为CC组(29例)、GC组(56例)和GG组(50例)。收集所有受试者的一般临床资料(年龄、性别、合并基础疾病情况)、实验室检查结果(IL-6、TNF-α、PCT)及IL-6基因启动子区-572C/G多态性检测结果并分组进行比较。采用χ^(2)检验分析基因型分布是否符合Hardy-Weinberg遗传平衡,采用logistic回归分析探讨IL-6基因启动子区-572C/G基因型与脓毒症的相关性。结果试验组患者IL-6、TNF-α和PCT水平均明显高于对照组,脓毒症休克组患者上述指标水平均明显高于脓毒症组;试验组患者IL-6基因启动子区-572C/G基因型CC及等位基因C频率明显低于对照组,基因型GG及等位基因G频率明显高于对照组;脓毒症休克组患者IL-6基因启动子区-572C/G基因型CC及等位基因C频率明显低于脓毒症组,基因型GG及等位基因G频率明显高于脓毒症组(P<0.05)。Logistic回归分析结果显示,等位基因模型基因型GG是CC患病风险的2.347倍,显性模型基因型GG+CG是CC患病风险的1.809倍,隐性模型基因型GG是CC+CG患病风险的1.830倍;IL-6基因启动子区-572C/G多态性(CC、CG、GG)为影响脓毒症发生的独立因素(P<0.05)。CC组患者IL-6、TNF-α和PCT水平均低于CG组和GG组(P<0.05)。结论IL-6基因启动子区-572C/G多态性与脓毒症的发病风险相关,且与脓毒症的进展显著相关,其多态性影响PCT和炎性因子IL-6、TNF-α的表达水平。
Objective To explore the relationship between peripheral blood interleukin 6(IL-6),tumor necrosis factor(TNF)-α and procalcitonin(PCT) levels with interleukin-6 gene promoter region-572C/G polymorphism in sepsis patients.Methods A total of 135 patients with sepsis admitted to Taihe Hospital Affiliated to Hubei College of Medicine from October 2018 to October 2020 were retrospectively included as the experimental group.According to whether shock occurred during hospitalization, the patients in experimental group were divided into sepsis group(63 cases) and sepsis shock group(72 cases).148 healthy people in our hospital during the same period were selected as the control group.According to genotype, patients in experimental group were divided into CC group(29 cases),GC group(56 cases) and GG group(50 cases).General clinical data(age, gender and underlying diseases),laboratory test results(IL-6,TNF-α and PCT) and IL-6 gene promoter region-572C/G polymorphism test results of all subjects were collected and grouped for comparison.χ^(2) test was used to analyze whether genotype distribution was consistent with Hardy-Weinberg genetic balance.Logistic regression analysis was used to investigate the correlation between IL-6 gene promoter region-572C/G genotype and sepsis.Results IL-6,TNF-α and PCT levels in experimental group were significantly higher than those in control group, and those in sepsis shock group were significantly higher than those in sepsis group;the frequencies of IL-6 gene promoter region-572C/G genotype CC and allele C in experimental group were significantly lower than those in control group, while the frequencies of genotype GG and allele G in experimental group were significantly higher than those in control group;the frequencies of IL-6 gene promoter region-572C/G genotype CC and allele C in sepsis shock group were significantly lower than those in sepsis group, while the frequencies of genotype GG and allele G in sepsis shock group were significantly higher than those in sepsis group(P<0.05).Logistic regression analysis showed that the allelic model genotype GG was 2.347 times the risk of CC,the dominant model genotype GG+CG was 1.809 times the risk of CC,and the recessive model genotype GG was 1.830 times the risk of CC+CG,The polymorphism of IL-6 gene promoter region-572C/G(CC、CG、GG) were independent factors affecting the occurrence of sepsis(P<0.05).IL-6,TNF-α and PCT levels in CC group were lower than those in CG group and GG group(P<0.05).Conclusion The IL-6 gene promoter region-572C/G polymorphism is associated with the risk of sepsis and significantly associated with the progression of sepsis, and its polymorphism affects the expression levels of PCT and inflammatory factors IL-6 and TNF-α.
作者
刘杰
陈森
Liu Jie;Chen Sen(Department of Intensive Care Medicine,Taihe Hospital affiliated to Hubei University of Medicine,Shiyan 442000,China;不详)
出处
《临床内科杂志》
CAS
2023年第3期167-170,共4页
Journal of Clinical Internal Medicine
基金
湖北省十堰市科技项目(X201910929032)
湖北医药学院教学研究课题(2019031)。
