摘要
目的:研究鹿茸多肽(PAP)对缺铁性贫血(IDA)大鼠的治疗作用并探讨其可能机制。方法:使用缺铁饲料诱导IDA大鼠模型,将40只IDA大鼠随机分为模型组(灌胃等体积的生理盐水),Low-PAP组、Medium-PAP组和High-PAP组(分别灌胃30、60和120 mg/kg的PAP),每组10只,另选取10只正常饲料喂养的同龄健康大鼠作为对照组(灌胃等体积的生理盐水)。每天灌胃1次,疗程为4周。末次给药24 h后,测量各组大鼠的体重、肝脏和脾脏指数,对肝脏和脾脏组织进行苏木素伊红(HE)染色。通过ELISA法检测血红蛋白(Hb)和促红细胞生成素(EPO)含量,通过比色法检测血清铁(SI)含量,通过透射电子显微镜观察肝脏和脾脏线粒体超微结构。使用相应试剂盒检测血清氧化应激指标水平。通过Western blot检测骨髓转铁蛋白受体(TFR)蛋白表达水平。结果:与模型组相比,Low-PAP组、Medium-PAP组和High-PAP组大鼠体重均升高,肝脏和脾脏指数均降低(P<0.05),且大鼠的肝脏和脾脏形态和线粒体超微结构明显改善,Hb、EPO和SI水平均升高(P<0.05)。与模型组相比,Low-PAP组、Medium-PAP组和High-PAP组大鼠的血清超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-PX)水平均升高,而丙二醛(MDA)降低(P<0.05)。与模型组相比,Low-PAP组、Medium-PAP组和High-PAP组大鼠的骨髓TFR蛋白相对表达量降低(P<0.05)。结论:本研究表明PAP可有效减轻IDA大鼠的临床症状,促进红细胞生成,增强造血功能,改善铁代谢,提高抗氧化能力,促进线粒体合成。
Objective:To investigate the therapeutic effect of pilose antler polypeptide(PAP)on iron deficiency anemia(IDA)rats and its possible mechanism.Methods:The IDA rat model was induced by iron deficiency diet.40 IDA rats were randomly divided into model group(intragastric administration of normal saline),Low-PAP group,Medium-PAP group and High-PAP group(intragastric administration of 30,60 and 120 mg/kg PAP,respectively),with 10 rats in each group.Another 10 healthy rats of the same age fed with normal diet were selected as control group.The rats were given intragastric administration once a day for 4 weeks.24 hours after the last administration,the body weight,liver and spleen indices of the rats in each group were measured,and the liver and spleen tissues were stained with hematoxylin and eosin(HE).Hemoglobin(Hb)and erythropoietin(EPO)contents were detected by ELISA,serum iron(SI)contents were detected by colorimetric method,and the ultrastructure of liver and spleen mitochondria was observed by transmission electron microscope.Serum oxidative stress indexes were detected with corresponding kits.The expression level of bone marrow transferrin receptor(TFR)protein was detected by Western blot.Results:Compared with that in the model group,the body weight of the rats in Medium-PAP group and High-PAP group increased,while the liver and spleen indexes decreased(P<0.05).Compared with that in the model group,the liver and spleen morphology and mitochondrial ultrastructure of Low-PAP group,Medium-PAP group and High-PAP group improved significantly,and the Hb,EPO and SI improved significantly(P<0.05).Compared with that in the model group,the levels of serum superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GSH-PX)in Low-PAP group,Medium-PAP group and High-PAP group all increased,while malondialdehyde(MDA)decreased(P<0.05).Compared with that in the model group,the relative expression of TFR protein in the bone marrow of the rats in Low-PAP group,Medium-PAP group and High-PAP group decreased(P<0.05).Conclusion:PAP can effectively alleviate the clinical symptoms of IDA rats,promote erythropoiesis,enhance hematopoietic function,improve iron metabolism,enhance antioxidant capacity,and promote mitochondrial synthesis.
作者
贾卫静
王健红
原瑞凤
程璐
段晓晖
JIA Wei-jing;WANG Jian-hong;YUAN Rui-feng;CHENG Lu;DUAN Xiao-hui(Department of Hematology,The First Affiliated Hospital of Air Force Medical University,Xi'an,Shaanxi,710032,China)
出处
《现代生物医学进展》
CAS
2023年第6期1027-1032,共6页
Progress in Modern Biomedicine
基金
陕西省自然科学基础研究计划项目(2021JM-241)。
关键词
缺铁性贫血
鹿茸多肽
铁代谢
造血功能
Iron deficiency anemia
Pilose antler polypeptide
Iron metabolism
Hematopoietic function
