摘要
为了解饥饿胁迫对鳙肌肉质构特性、常规营养成分、氨基酸组成及肠道微生物群落结构的影响,设置正常投喂组和饥饿组,对处于越冬前期的鳙(Aristichthys nobilis)开展78 d饥饿试验。结果表明,与正常投喂组相比,饥饿组鳙肌肉的硬度、黏性、咀嚼性、恢复性及水分含量显著提高,肌肉粗蛋白、粗脂肪及氨基酸含量显著降低(P<0.05);2组间鳙肌肉弹性、灰分、蛋氨酸含量以及必需氨基酸、非必需氨基酸、鲜味氨基酸总量占氨基酸总量的百分比差异不显著(P>0.05)。此外,2组间肠道微生物群落结构差异显著,正常投喂组鳙肠道内容物菌群的Alpha多样性显著高于饥饿组(P<0.05);在门水平上,正常投喂组优势菌群为变形菌门、放线菌门、蓝细菌门和拟杆菌门,饥饿组优势菌群为梭杆菌门、厚壁菌门、蓝细菌门、变形菌门和放线菌门;正常投喂组鱼变形菌门、放线菌门的比例显著高于饥饿组,梭杆菌门、厚壁菌门显著低于饥饿组(P<0.05);饥饿组肠道鲸杆菌属、迪尔玛菌属、蓝细菌属的丰度显著增加(P<0.05)。指出,越冬前长期饥饿,会导致肌肉营养成分含量较少,肠道菌群多样性降低,菌群结构发生显著改变。
To investigate the effects of starvation on muscle textural properties,conventional nutritive composition,amino acids content and intestinal microbiota of early wintering Aristichthys nobilis,fish were selected for a 78-day fasting treatment meanwhile the control group fed with commercial food normally.The results showed that starvation caused a significant increase in the hardness,gumminess,chewiness,resilience and moisture of muscle,and a significant decrease in the crude protein,crude fat and amino acid content of muscle(P<0.05);the difference of springiness,ash and methionine between two groups weren’t significant(P>0.05).Moreover,we observed lower intestinal microbial diversity in starvation group(P<0.05);intestinal dominant phyla in feeding group were Proteobacteria,Actinobacteriota,Cyanobacteria and Bacteroidota,while in starvation group were Fusobacteriota,Firmicutes,Cyanobacteria,Proteobacteria and Actinobacteriota,the relative abundance of Proteobacteria and Actinobacteriota were significantly decreased while Fusobacteriota and Firmicutes were increased in starvation group(P<0.05);intestinal dominant genus showed that the relative abundance of Cetobacterium,Dielma and Cyanobium PCC-6307 were significantly increased in the latter(P<0.05).Our results suggested that starvation could induce lower levels of muscle nutrients,decrease intestinal microbiota diversity and alter microbiota structure.
作者
万金娟
张国清
沈美芳
林海
张美琴
邵俊杰
WAN Jinjuan;ZHANG Guoqing;SHEN Meifang;LIN Hai;ZHANG Meiqin;SHAO JunJie(Freshwater Fisheries Research Institute of Jiangsu Province,Nanjing,Jiangsu 210017,China;Jiangsu Vocational Institute of Commerce,Nanjing,Jiangsu 211168,China)
出处
《水产养殖》
CAS
2023年第5期1-8,共8页
Journal of Aquaculture
基金
国家现代农业产业技术体系资金(CARS-46)
江苏现代农业(大宗鱼类)产业技术体系(JATS[2022]408)。
关键词
鳙
饥饿胁迫
肌肉品质
肠道菌群
Aristichthys nobilis
Starvation
Muscle quality
Intestinal microbiota
