摘要
目的建立定量检测地肤子中9种成分含量的高效液相色谱(HPLC)法,并联合化学计量学及灰色关联度分析对其质量控制进行研究。方法采用HPLC法同时检测地肤子中芦丁、金丝桃苷、异鼠李素-3-O-葡萄糖苷、槲皮素、异鼠李素、齐墩果酸、胡萝卜苷、地肤子皂苷Ⅰc、β-谷甾醇含量,并通过聚类分析、主成分分析、正交偏最小二乘法-判别分析及灰色关联度分析比较不同产地地肤子质量差异。结果芦丁、金丝桃苷、异鼠李素-3-O-葡萄糖苷、槲皮素、异鼠李素、齐墩果酸、胡萝卜苷、地肤子皂苷Ⅰc和β-谷甾醇分别在各自范围内线性关系良好(r>0.9992);精密度、稳定性及重复性良好;平均加样回收率在96.93%~100.12%。聚类分析结果显示17批样品聚为3类;主成分分析结果显示主成分1、2是影响样品质量评价的主要因子;正交偏最小二乘法-判别分析结果显示地肤子皂苷Ⅰc、芦丁和齐墩果酸变量重要性投影(VIP)值均>1,是影响产品质量差异的标志性成分;灰色关联度分析结果显示17批样品的相对关联度在0.3340~0.6225,不同产地地肤子质量存在一定差异。结论所建立的HPLC多组分定量联合化学计量学及灰色关联度分析方法操作便捷、结果准确,可为地肤子质量控制和品质评价提供依据。
Objective To establish an HPLC method for simultaneous determination of nine components in Kochia scoparia,and to study the quality by combining chemometrics and grey relational analysis(GRA).Method HPLC method was used to simultaneously determine the contents of rutoside,hyperoside,isorhamnetin-3-O-beta-D-glucoside,quercetin,isorhamnetin,oleanolic acid,eleutheroside A,kochioside Ic andβ-sitosterol in K.scoparia.The quality differences of K.scoparia from different producing areas were compared by cluster analysis,principal component analysis,orthogonal partial least-squares methoddiscriminant analysis and grey correlation analysis.Results The linear relationship of rutoside,hyperoside,isorhamnetin-3-O-betaD-glucoside,quercetin,isorhamnetin,oleanolic acid,eleutheroside A,kochioside Ic andβ-sitosterol was good in their respective ranges(r>0.9992).The method had good precision,stability,and repeatability(RSD<2.0%).The average recovery(n=9)was between 96.93%and 100.12%(RSD<2.0%).Cluster analysis showed that 17 batches of K.scoparia were clustered into three categories.Principal component analysis showed that principal component 1 and 2 was the main factor affecting the quality evaluation of the samples.Orthogonal partial least squares-discriminant analysis showed that VIP values of kochioside Ic,rutoside and oleanolic acid were all greater than 1,which were the symbolic components affecting the quality difference of products.GRA showed that the relative correlation degree of 17 batches of K.scoparia was between 0.3340 and 0.6225.There were some differences in the quality of K.scoparia from different producing areas.Conclusion The established HPLC multi-component quantitative method combined with chemometrics and grey relational analysis is convenient and accurate,which can provide a basis for quality control and quality evaluation of K.scoparia.
作者
乔乐天
刘源
贾号
孙彬
QIAO Letian;LIU Yuan;JIA Hao;SUN Bin(Department of Pharmacy,The First People's Hospital of Shangqiu,Shangqiu 476100,China;Institute of Biopharmaceutical Research,Liaocheng University,Liaocheng 252059,China)
出处
《药物评价研究》
CAS
2023年第4期812-820,共9页
Drug Evaluation Research
基金
国家自然科学基金青年科学基金项目(81703357)
河南省医学科技攻关计划联合共建项目(LHGJ20210992)。
