摘要
为研究贮藏温度和贮藏时间对蜂王浆品质的影响,以椴树浆、油菜浆、洋槐浆3种不同来源的蜂王浆为原料,通过测定其总蛋白、10-羟基-2-癸烯酸(10-HDA)、游离糖、游离氨基酸和游离脂肪酸含量,同时分析其羟自由基和DPPH自由基清除能力的变化规律。结果表明,不同蜜源蜂王浆之间总蛋白含量和10-HDA含量存在显著差异,短期贮藏不会导致其发生显著变化,但会导致油菜浆中葡萄糖和果糖等单糖含量的升高以及蔗糖含量的显著减少(P<0.05)。不同花粉源蜂王浆中氨基酸和游离脂肪酸组成类似,短期贮藏后油菜浆中的谷氨酸、棕榈酸、油酸、亚油酸和α-亚麻酸显著增加,椴树浆中丙氨酸、油酸及洋槐浆中亮氨酸和组氨酸则出现降低的现象。3种不同来源的蜂王浆的羟自由基和DPPH自由基清除能力在贮藏后显著降低(P<0.05)。本研究可以为蜂王浆在化学成分及抗氧化活性变化等方面的品质评价提供理论参考。
To study the effect of storage temperature and storage time on the quality of royal jelly,the contents of total protein,10-HDA,free sugar,free amino acid and free fatty acid of royal jelly were determined on linden tree royal jelly,cole royal jelly and robinia royal jelly.The scavenging ability of hydroxyl radical and DPPH radical were also analyzed.The results showed that there were significant differences in total protein content and 10-HDA content among different nectar sources.Short-term storage did not cause significant changes in total protein content and 10-HDA content,but the content of glucose and fructose of cole royal jelly increased and the content of sucrose decreased significantly(P<0.05).The contents of amino acids and free fatty acids were similar.After short-term storage,the contents of glutamic acid,palmitic acid,oleic acid,linoleic acid andα-linolenic acid in cole royal jelly were significantly increased,while alanine,oleic acid in linden tree royal jelly and leucine and histidine in robinia royal jelly were decreased.The scavenging abilities of hydroxyl radical and DPPH free radical in three kinds of royal jelly from different sources were significantly decreased after storage(P<0.05).This study can provide theoretical reference for the quality evaluation of royal jelly in chemical composition and antioxidant activity.
作者
肖立涵
辛美果
卢文静
叶沁
张岑
肖朝耿
谌迪
XIAO Lihan;XIN Meiguo;LU Wenjing;YE Qin;ZHANG Cen;XIAO Chaogeng;CHEN Di(College of Food Science and Engineering,Foshan University,Foshan 528231,Guangdong,China;Institute of Food Science,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)
出处
《浙江农业学报》
CSCD
北大核心
2023年第5期1161-1167,共7页
Acta Agriculturae Zhejiangensis
基金
国家自然科学基金(32201930)
浙江省重点研发计划项目(2019C02069)。
关键词
蜂王浆
贮藏
温度
品质
抗氧化能力
royal jelly
storage
temperature
quality
antioxidant activity