摘要
为研究两种糖水平下α-硫辛酸(α-LA)对日本沼虾肠道菌群的影响,以玉米淀粉为糖源,配制2×3因子的试验饲料,即2种糖水平(15%和30%)、3种α-LA浓度(0、700和1400 mg/kg),将平均体重为(0.050±0.003) g的1 500尾日本沼虾幼虾随机分为6组,每组5个平行,进行为期8周的饲养试验.结果表明:在相同α-LA下,30%糖水平组日本沼虾肠道菌群的Chao指数和Ace指数显著低于15%糖水平组(P<0.05),但在30%糖水平下,随着α-LA的增加,Chao指数和Ace指数显著升高(P<0.05);日本沼虾肠道菌群主要以厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)、软壁菌门(Tenericutes)和放线菌门(Actinobacteria)4个门为主,糖水平和α-LA浓度对其丰度具有显著的交互作用(P<0.05),在15%糖水平下,摄食700 mg/kg α-LA组的变形菌门和软壁菌门的相对丰度显著高于其余两组(P<0.05);从属水平上分析,日本沼虾肠道菌群主要以Tyzzerella_3属、肠杆菌属(Enterobacter)、红球菌属(Rhodococcus)、气单胞菌属(Aeromonas)和假单胞菌属(Pseudomonas)5个属为主,肠杆菌属主要受α-LA浓度的影响,而气单胞菌属主要受糖水平影响.由此可见,高糖能降低肠道菌群的丰度,α-LA的添加可缓减高糖对肠道菌群相对丰度的抑制作用,在两种糖水平下添加700 mg/kg α-硫辛酸,可改善日本沼虾肠道菌群的结构和稳定性.
In order to investigate the effects of alpha lipoic acid(α-LA)on the intestinal microflora of oriental river prawn Macrobrachium nipponense,six diets were formulated using corn starch as the carbohydrate source,at two carbohydrate levels(15%and 30%)and three concentrations ofα-LA supplementation(0,700,and 1400 mg/kg).Each experimental diet was fed to prawns(mean weight 0.050±0.003 g),twice daily to apparent satiation,in five replicates.The feeding trial lasted for 8 weeks.The results are as follows:(1)At the sameα-LA concentration,the Chao and Ace indexes in 30%(high)-carbohydrate groups were significantly lower than that of 15%(low)-carbohydrate groups.But the Chao and Ace indexes increased with the increase ofα-LA in the 30%(high)-carbohydrate groups.(2)At the phylum level,there were 4 dominant bacteria phyla in the intestinal tract,including Firmicutes,Proteobacteria,Tenericutes and Actinobacteria.There was significant interaction between the carbohydrate andα-LA levels and abundance of gut microflora(P<0.05).Under 15%carbohydrate level,the abundance of Proteobacteria and Tenericutes in prawns fed 700 mg/kgα-LA was significantly higher than that of other two groups,while under 30%carbohydrate level,the abundance of Firmicutes in prawns fed 700 mg/kgα-LA was significantly higher than that of other two groups.(3)At the genus level,there were 5 dominant bacteria genera in the intestinal tract,including Tyzzerella_3,Enterobacter,Rhodococcus,Aeromonas and Pseudomonas.Enterobacter was mainly affected byα-LA concentration,while Aeromonas was mainly affected by carbohydrate level.Thus,α-LA supplementation can alleviate the negative effect of reduced abundance by high carbohydrate,and 700 mg/kgα-LA supplementation under either dietary carbohydrate level could improve the stability of intestinal florain of M.nipponense.
作者
李柒梅
熊云凤
薛岩松
程先慰
韩星宇
李小丽
丁志丽
LI Qimei;XIONG Yunfeng;XUE Yansong;CHENG Xianwei;HAN Xingyu;LI Xiaoli;DING Zhili(Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development,Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition of Chinese Academy of Fishery Sciences,School of Life Sciences,Huzhou University,Huzhou 313000,China)
出处
《湖州师范学院学报》
2023年第4期33-43,共11页
Journal of Huzhou University
基金
国家自然科学基金项目(31402308)
国家级大学生创新创业训练计划项目(202110347046)。
关键词
糖
甲壳动物
Α-硫辛酸
肠道健康
carbohydrate
crustacean
α-lipoic acid
intestinal health
