摘要
水稻萌发的分蘖具有发育成完整水稻植株的潜能,是进行水稻大田无性繁殖的重要储备。本研究旨在分析比较3个不同籼、粳稻类型雌性不育系的剥蘖快繁和迭代再生力特性。结果表明,据籼粳遗传组分分类‘云岭314FS’、‘云岭319FS’为(偏)粳型;‘云岭317FS’为(偏)籼型。粳型雌性不育系‘云岭314FS’、‘云岭319FS’在继代(0~5次)和间隔时间(20,30,40 d)处理下的迭代繁殖系数显然高于籼型‘云岭317FS’;‘云岭314FS’、‘云岭319FS’的再生力明显优于‘云岭317FS’,叶绿素含量衰减率也相较于‘云岭317FS’低;3个雌性不育系的再生力随继代次数递增而逐渐降低,叶绿素含量衰减率则逐渐增加;‘云岭314FS、云岭317FS’再生力随剥蘖间隔时间加大而逐渐加大,叶绿素含量衰减率随间隔时间加大而减小,‘云岭319FS’再生力在间隔时间处理间相差较小且相对稳定。本研究初步探索出一套剥蘖繁殖雌性不育系的高效无性快繁技术体系,为FM-Line制种技术的推广奠定了基础,也为无性繁殖技术提供新的方法。
The rice tiller is a reserve for asexual propagation of rice in the field,and it has the potential to develop into a complete plant.This study was aimed to identify characterization of rapid tiller propagation and iterative regeneration potential in 3 rice female sterile lines with different indica-japonica genetic background.The result reveals that:according to the indica-japonica genetic components of 3 female sterile lines,'YN314FS'and'YN319FS'are classified as japonica(inclined)type,whileas‘YN317FS'is classified as indica(inclined)type;the iterative propagation coefficients of YN314FS and YN319FS are significantly higher than that of YN317FS under treatments of interval time(20~30~40 days)and iterations(0~5 th);the regenerative potential of YN314FS and YN319FS are obviously superior to that of YN317FS,and the decline rate of SPAD value is also lower than that of YN317FS;the regenerative potential of the three female sterile lines decreased significantly with the increase in iterations,and the decline rate of chlorophyll increased significantly;the regenerative potential of YN314FS and YN317FS increased significantly with the increase of the interval time,and the decline rate of chlorophyll decreased with the increase of the interval time,in addition,the regenerative potential of YN319FS has a small and relatively stable difference in interval time.This research has initially explored a set of asexual and efficient rapid propagation technology system for reproducing female sterile lines by tiller propagation,which is the basis of FM-line hybrid seed production technology,and replenish a novel method of asexual reproduction technology.
作者
李仕川
刘福根
王天杰
王镜博
罗迪
余卫霖
吴文彬
郭效琼
李伟
朱骞
李东宣
陈丽娟
Li Shichuan;Liu Fugen;Wang Tianjie;Wang Jingbo;Luo DiYu Weilin;Wu Wenbin;Guo Xiaoqiong;Li Wei;Zhu Qian;Lee Dongsun;Chen Lijuan(Rice Research Institute,Yunnan Agricultural University,Kunming,650201;The Key Laboratory for Crop Production and Intelligent Agriculture of Yunnan Province,Yunnan Agricultural University,Kunming,650201;State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Yunnan Agricultural University,Kunming,650201)
出处
《分子植物育种》
CAS
北大核心
2023年第9期2999-3008,共10页
Molecular Plant Breeding
基金
云南省重大专项(2018ZG005)
国家自然科学基金项目(31860108
31560115)
国家重点研发项目(YFD-0101101-5)
2017云岭英才计划-‘云岭高端外国专家’项目共同资助。
关键词
水稻
雌性核不育系
剥蘖繁殖
再生力
Rice(Oryza sativa L.)
Female sterile lines
Tiller propagation
Regenerative potential
