摘要
目的:观察荣筋拈痛方对膝骨关节炎大鼠软骨组织自噬的影响,探讨其防治膝骨关节炎的作用机制。方法:将24只SD大鼠按照随机分层法分为假手术组、模型对照组、荣筋拈痛方组,每组8只。除假手术组外,其他组采用改良Hulth法复制膝骨关节炎模型。给药12周后,从假手术组和模型对照组中随机抽取3只大鼠拍摄膝关节MRI,观察模型是否成功。采集大鼠膝关节组织,HE染色法观察软骨组织形态结构,采用Mankin病理评分对关节软骨进行组织形态学评分;免疫组织化学法观察软骨组织酵母自噬相关基因6同源物(Beclin1)及微管相关蛋白Ⅰ轻链3B(LC3B)的定位与表达。结果:MRI显示,假手术组关节间隙正常,关节面平整;模型对照组关节间隙增宽,关节软骨面不平整、局部缺损,结缔组织增生,骨赘形成。荣筋拈痛方组大鼠骨关节炎模型关节软骨的大体外观及HE染色均改善明显,Mankin软骨评分高于模型对照组,差异有统计学意义(P<0.01)。荣筋拈痛方组自噬蛋白Beclin1及LC3B表达均呈上升趋势。结论:荣筋拈痛方可能通过提高自噬相关蛋白Beclin1、LC3B的表达,改善软骨细胞状态,延缓关节软骨退变。
Objective:To observe the effect of Rongjin Niantong Fang(荣筋拈痛方)on autophagy of cartilage tissue in rats with knee osteoarthritis,and explore its mechanism of prevention and treatment of knee osteoarthritis.Methods:Twenty four SD rats were randomly stratified into a sham operation group,a model control group,and a Rongjin Niantong Fang group,with 8 rats in each group.Except the sham operation group,other groups used the improved Hulth method to replicate knee osteoarthritis models.After 12 weeks of administration,3 rats were randomly selected from the sham operation group and the model control group to take MRI images of knee joint and observe whether the model was successful.Collect knee joint tissue from rats,observe the morphology and structure of cartilage tissue using HE staining method,and score the tissue morphology of joint cartilage using Mankin pathological score;immunohistochemical method was used to observe the localization and expression of yeast autophagy related gene 6 homolog(Beclin1)and microtubule associated protein I light chain 3B(LC3B)in cartilage tissue.Results:MRI showed that the joint space in the sham operation group was normal and the joint surface was flat;in the model control group,the joint space widened,the articular cartilage surface was uneven with local defects,and there were connective tissue hyperplasia,and osteophyte formation.The general appearance and HE staining of the articular cartilage in the Rongjin Niantong Formula group of rats with osteoarthritis model improved significantly,and the Mankin cartilage score was higher than the model control group,with a statistically significant difference(P<0.01).The expression of autophagy proteins Beclin1 and LC3B in the Rongjin Niantong Fang group showed an upward trend.Conclusion:Rongjin Niantong Fang may improve the state of chondrocytes and delay joint cartilage degeneration by increasing the expression of autophagy related proteins Beclin1 and LC3B.
作者
李路
王文义
潘丹虹
林晴
付长龙
郑春松
马德尊
叶锦霞
LI Lu;WANG Wen-yi;PAN Dan-hong;LIN Qing;FU Chang-long;ZHENG Chun-song;MA De-zun;YE Jin-xia
出处
《风湿病与关节炎》
2023年第5期1-5,11,共6页
Rheumatism and Arthritis
基金
福建省自然科学基金项目(2020J01749)
福建中医药大学陈可冀中西医结合发展基金项目(CKJ2021015)。
关键词
膝骨关节炎
荣筋拈痛方
软骨组织
自噬
大鼠
knee osteoarthritis
Rongjin Niantong Fang(荣筋拈痛方)
cartilage tissue
autophagy
rats
