摘要
目的 探讨慢性萎缩性胃炎(chronic atrophic gastritis,CAG)患者血清白三烯B4(leukotriene B4,LTB4)和CX3C趋化因子配体1(CX3C ligand 1,CX3CL1)表达水平及其诊断价值。方法 研究对象为2018年3月~2021年3月忻州市中医医院收治的98例CAG患者,另选取同期80例健康体检者为对照组。采用酶联免疫法(enzyme-linked immunosorbent assay,ELISA)测定血清LTB4和CX3CL1水平,并分析两者与患者临床病理特征的关系。采用Pearson法分析CAG患者血清中LTB4与CX3CL1表达的相关性,采用Logistic回归分析影响该疾病发生的危险因素。受试者工作特征(receiver operating characteristic,ROC)曲线分析血清LTB4和CX3CL1水平对CAG患者的诊断价值。结果与对照组相比,CAG患者血清中LTB4(8.15±2.13 pg/ml vs 5.89±1.26 pg/ml)和CX3CL1(132.06±30.59 pg/ml vs 100.26±12.61 pg/ml)表达均明显升高,差异具有统计学意义(t=8.368,8.710,均P <0.05);LTB4和CX3CL1表达水平与患者年龄、性别、Hp感染无关(χ^(2)=1.521~3.397,均P>0.05),与慢性炎症、胃黏膜萎缩、肠化生、不典型增生有关(χ^(2)=4.083~11.975,均P<0.05)。Pearson法分析显示,CAG患者血清中LTB4与CX3CL1表达呈正相关(r=0.235,P=0.020)。Logistic回归分析结果显示,血清LTB4(OR=1.777,95%CI=1.398~2.259)和CX3CL1(OR=1.054,95%CI=1.031~1.077)是影响CAG发生的独立危险因素(均P<0.05)。ROC曲线分析结果显示,LTB4和CX3CL1及二者联合检测诊断CAG患者的AUC分别为0.805,0.822和0.877,二者联合诊断效能优于血清LTB4和CX3CL1各自单独诊断(Z二者联合-LTB4=3.031,P=0.024;Z二者联合-CX3CL1=2.340,P=0.019)。结论 CAG患者血清中LTB4和CX3CL1均呈高表达,是影响CAG发生的独立危险因素,且二者联合检测的诊断价值较高,可作为判断CAG病情活动期的观察指标。
Objective To investigate the levels of serum leukotriene B4(leukotriene B4,LTB4)and CX3C chemokine ligand 1(CX3C ligand 1,CX3CL1)in patients with chronic atrophic gastritis(chronic atrophic gastritis,CAG)and their diagnostic value.Methods The study objects were 98 patients with CAG admitted to Xinzhou Hospital of Traditional Chinese Medicine from March 2018 to March 2021,and 80 healthy subjects in the same period were selected as the control group.Enzyme-linked immunosorbent assay(ELISA)was applied to measure serum LTB4 and CX3CL1 levels,and the relationship between the two and the clinicopathological characteristics of patients was analyzed.Pearson method was applied to analyze the correlation between the expression of LTB4 and CX3CL1 in serum of CAG patients.Logistic regression was applied to analyze the risk factors of the disease,and receiver operating characteristic(ROC)curve was used to evaluate the diagnostic value of serum LTB4 and CX3CL1 levels in patients with CAG.Results Compared with the control group,the expressions of LTB4(8.15±2.13 pg/ml vs 5.89±1.26 pg/ml)and CX3CL1(132.06±30.59 pg/ml vs 100.26±12.61 pg/ml)in serum of CAG patients were greatly increased and the differences were statistically significant(t=8.368,8.710,all P<0.05).The expression levels of LTB4 and CX3CL1 were not related to age,gender and Hp infection of patients(χ^(2)=1.521~3.397,all P>0.05),but were related to chronic inflammation,gastric mucosal atrophy,intestinal metaplasia and atypical dysplasia(χ^(2)=4.083~11.975,all P<0.05).Pearson analysis showed that the expressions of LTB4 and CX3CL1 in serum of CAG patients were positively correlated(r=0.235,P=0.020).Logistic regression analysis showed that serum LTB4(OR=1.777,95%CI=1.398~2.259)and CX3CL1(OR=1.054,95%CI=1.031~1.077)were independent risk factors for the occurrence of CAG(all P<0.05).The results of ROC curve analysis showed that the AUC of LTB4,CX3CL1 and their combined detection in CAG patients was 0.805,0.822 and 0.877,respectively.The combined diagnostic efficiency of the two was better than that of serum LTB4 and CX3CL1 alone(Zcombination-LTB4=3.031,P=0.024;Zcombination-CX3CL1=2.340,P=0.019).Conclusion LTB4 and CX3CL1 were highly expressed in serum of CAG patients,which would be independent risk factors for the occurrence of CAG.The combined detection of the two has q high diagnostic value,which can be used as an observation index to judge the active period of CAG disease.
作者
史秋霞
李瑞
邸亚星
SHI Qiu-xia;LI Rui;DI Ya-xing(Department of Gastroenterology,Xinzhou Hospital of Traditional Chinese Medicine,Shanxi Xinzhou 034000,China)
出处
《现代检验医学杂志》
CAS
2023年第3期149-153,共5页
Journal of Modern Laboratory Medicine
