摘要
5型磷酸二酯酶(PDE-5)是生物体内专一水解细胞内环磷酸鸟苷(cGMP)的关键因子,具有调节血管张力、传导视觉信号、调控能量代谢等作用。5型磷酸二酯酶抑制剂(PDE-5i)作为选择性抑制磷酸二酯酶活性的一类药物,可用于改善勃起功能障碍、治疗肺动脉高压等疾病,在临床上广泛应用。一些不法商家向保健食品中非法添加PDE-5i及其类似物以达到其声称的保健效果,严重危害消费者健康。本文综述了保健食品中非法添加PDE-5i及其类似物的常用检测方法,包括理化快速筛查法、酶联免疫法、胶体金免疫层析法、近红外光谱法、拉曼光谱法、离子迁移谱法、薄层色谱及其联用法、液相色谱法、液相色谱-质谱联用法等,并介绍了各个方法的特点,为市场监管部门和公安机关打击食品药品违法犯罪行为提供技术支撑。
People are pursuing quality of life higher and higher,bringing forth the ever-growing demand for healthcare food.Driven by interests,some healthcare-food enterprises even add illegal drugs like sildenafi l(categorized to the PDE-5i described below)into their products,posing a serious threat to the health and life safety of consumers.State Council’s Offi ce of the Food Safety Committee issued“Notice on further strengthening supervision into quality and safety of healthcare foods”,requiring severe crack-down on those illegal behaviors and protection to the health of consumers.Therefore,the Offi ce of State Food and Drug Administration announced“List of substances potential of illegally added into healthcare foods(fi rst batch)”in 2012,concluding the products that claim to be functional of relieving physical fatigue(anti-fatigue)and enhancing immunity(modulating immunity)may be illegally adulterated with the substances of red denafi l,vardenafi l,hydroxyhomosildenafi l,sildenafi l,homosildenafi l,aminotadalafi l,tadalafi l,thiophanate and the like.Accordingly,rapid and accurate detection of the drugs illegally added into healthcare food is of great signifi cance to keep and sustain the normal operation of healthcare food market and effectively prevent and combat against crimes involving with food and drug.Phosphodiesterase type 5(PDE-5)is a key factor in the hydrolysis of intracellular cyclic guanosine phosphate(cGMP)in vivo,participating in regulating vascular tension,transmitting visual signals and controlling energy metabolism.Phosphodiesterase type 5 inhibitor(PDE-5i),a class of drugs that selectively inhibit phosphodiesterase activity,can play role in improving erectile dysfunction,treating pulmonary hypertension and other diseases,hence making it widely used in clinical practice.At times,PDE-5i-analogous drugs have been illegally added into healthcare food under the guise of health effect.A review was here made about the common methods for detecting the illegally-added phosphodiesterase-5 inhibitors(PDE-5i)and analogues in healthcare food,having enumerated physical and chemical rapid screening,enzyme-linked immunosorbent assay(ELISA),gold immunochromatography assay(GICA),near infrared spectrometry(NIRS),surface enhanced Raman spectroscopy(SERS),ion mobility spectrometry(IMS),thin layer chromatography(TLC),high performance liquid chromatography(HPLC),liquid chromatography-mass spectrometry(LC-MS),and liquid chromatography-tandem mass spectrometry(LC-MS/MS).With introduction to the characteristics of each method indicated above,this article should be of providing technical support and reference for market regulating authorities and public security law enforcers to crack down on crimes about food and drug.
作者
李少元
接强
栾玉静
董颖
崔冠峰
黄健
孙晓宇
王芳琳
LI Shaoyuan;JIE Qiang;LUAN Yujing;DONG Ying;CUI Guanfeng;HUANG Jian;SUN Xiaoyu;WANG Fanglin(Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China)
出处
《刑事技术》
2023年第3期221-227,共7页
Forensic Science and Technology
基金
国家重点研发计划(2018YFC1602705)
中央级公益性科研院所基本科研业务费专项资金项目(2020JB020、2020JB026)。
