摘要
为了找到一种快速有效的脱黏方法,提升大刺鳅人工繁育的效率,使用3种脱黏剂:黄泥浆(A)、滑石粉(B)和木瓜酶(C)对大刺鳅受精卵进行脱黏,并将木瓜酶脱黏剂设4种配方:C1、C2、C3、C4,每个脱黏剂设4个脱黏时间:15、30、45、60 s,对比分析了3种脱粘剂对大刺鳅受精卵孵化效果的影响。结果显示:A组的孵化率为30.71%±4.41%,B组的孵化率为60.18%±10.12%,虽然滑石粉的脱黏效果优于黄泥浆,但二者效果均不佳;而木瓜酶作为新的脱黏剂,实验中最高孵化率组为C3-15 s组,孵化率高达96.26%±4.61%,其次是C_(3)-30 s组的孵化率为92.12%±3.32%。综上,选择适宜的脱黏配方和时间,可以有效提高大刺鳅受精卵的孵化率,其最佳脱黏配方为0.25%Na_(2)SO_(3)+0.125%L-半胱氨酸盐酸盐+0.05%木瓜酶,脱黏时间为15~30 s。
This paper aims to find a rapid and effective method of debonding and improve the efficiency of artificial breeding of Mastacembelus armatus.Yellow-earth slurry(A),talcum powder(B)and papain(C)were used for debonding fertilized eggs of Mastacembelus armatus,and the papain reagent was formulated in four recipes(C 1,C 2,C 3,C 4),and four debonding time(15s,30s,45s,60s)were set,then the effects of three debongding reagents on hatching were assessed.The results showed the effect of talcum powder was better than that of yellow-earth slurry,but both of them were not good,the hatch rate of A group and B group was 30.71%±4.41%and 60.18%±10.12%,respectively.Papain was a new debonding reagent,the highest hatching rate was C_(3)-15s group,which was 96.26%±4.61%,followed by C_(3)-30 s group,and the hatching rate was 92.12%±3.32%.Therefore,the hatching rate of the fertilized eggs can be effectively improved by controlling the debonding recipe and time appropriately,and the optimal debonding recipe is Na_(2)SO_(3)(0.25%)+L-cysteine monohydrochloride(0.125%)+papain(0.05%),the optimal debonding time is 15-30 s.
作者
刘银华
LIU Yinhua(Freshwater Fisheries Research Institute of Fujian,Fuzhou 350000)
出处
《中国农学通报》
2023年第14期133-138,共6页
Chinese Agricultural Science Bulletin
基金
福建省海洋与渔业结构调整专项资金“构建大刺鳅全同胞家系及育种值评估方法的研究”(2020MDS-YT005)
福建省省属公益类科研院所基本科研专项“大刺鳅全人工苗种繁育技术及性别分化机理研究”(2020R1014002)。
关键词
大刺鳅
受精卵
黏性卵
脱黏
黄泥浆
滑石粉
木瓜酶
Mastacembelus armatus
fertilized egg
adhesive egg
debonding
yellow-earth slurry
talcum powder
papain
